normalization.boxplot: Boxplot of gene expressions

Description Usage Arguments Details Value Author(s) References Examples

View source: R/normalization.boxplot.R

Description

Boxplot of the "log(ar+1)" transform of the data before and after the statistical functional depth based normalization. This is commonly carried out to show the adequacy of the procedure.

Usage

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normalization.boxplot(
  ar,
  namesN = T,
  before = F,
  after = T,
  par.r = F,
  par.c = F,
  arg1 = "Before",
  arg2 = "After"
)

Arguments

ar

Cell files from Affy micrarray experiment: a matrix where the columns are the sample elements, RNA-seq or microarray, and the rows the variables (the genes).

namesN

If TRUE, the labels of each RNA-seq's or microarray are substituted by a number corresponding to its position in data matrix. The default is TRUE.

before

If TRUE, the "log(ar+1)" transform of the data before the normalization step is plotted. The default is FALSE.

after

If TRUE, the "log(ar+1)" transform of the data after the normalization step is plotted. The default is TRUE.

par.r

If TRUE, and both "before" and "after" are also TRUE, the "before" and "after" boxplots are displayed in a row. The default is FALSE.

par.c

IIf TRUE, and both "before" and "after" are also TRUE, the "before" and "after" boxplots are displayed in a column. The default is FALSE.

arg1

Overall title for the boxplot before the normalization step. The default is Before.

arg2

Overall title for the boxplot after the normalization step. The default is After.

Details

Boxplot of the "log(ar+1)" transform of the data before and after the normalization step. This is commonly carried out to show the adequacy of the procedure.

Value

The boxplot of the "log( +1)" transform of the data before and/or after the normalization step.

Author(s)

A. Nieto and J. Cabrera

References

Nieto-Reyes A, Cabrera J. Statistical depth based normalization and outlier detection of gene expression data. Preprint.

Examples

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## Computes the normalization of the RNA-seq's in the Airway dataset.
N = normalization(Airway)

## Displays in a row the boxplots of the "log(+1)" transform 
## of the data before and after normalization.

normalization.boxplot(Airway, before = TRUE, par.r = TRUE)

asael697/fdaRNA documentation built on May 28, 2020, 11:32 a.m.