applyBandpass: Apply Bandpass Filter to Fluorescence Intensities

In bpollner/flowdex: Extract Fluorescence Distribution Data from FCS Files and Recalculate to Events per Volume

Apply Bandpass Filter to Fluorescence Intensities

Description

Apply a bandpass like filter to the fluorescence intensities within a single gate.

Usage

applyBandpass(fdmat, bandpass, gate = 1)

Arguments

fdmat	An object of class-fdmat as produced by makefdmat.
bandpass	Numeric vector length two, holding the lower and upper boundaries of the bandpass filter.
gate	Numeric or character length one, defining the gate on which the bandpass should be applied. Defaults to 1. (See 'fdmat@metadata' for possible values for gate name resp. number.)

Value

The same object of class 'fdmat' as was provided to the argument 'fdmat', but with modified range of fluorescence intensities in the gate specified under 'gate'.

See Also

Other Accessory functions: checkRepairFcsFiles(), cutFdmatToGate(), exportFdmatData(), fd_load(), fd_save(), genfs(), repairSID(), repairVolumes()

Examples

td <- tempdir()
data_source <- "https://github.com/bpollner/data/raw/main/flowdex_examples/flowdex_examples.zip"
check_download_data(td, data_source)
exp_home <- paste0(td, "/flowdex_examples")
old_wd <- getwd()
setwd(exp_home)
#
assign("get_settings_from_flowdex_package_root", TRUE, pos=.GlobalEnv)
# only required to make the examples run automatically
# you should not call 'assign' if you run the examples manually
# the effect of setting 'get_settings_from_flowdex_package_root' to TRUE
# is that the file 'flowdex_settings.R' in 'root' of the installed package
# 'flowdex' will be sourced instead of the one in the user-defined location.
#
fdmat <- flowdexit(patt = "GPos_T4")
fdmat_bp <- applyBandpass(fdmat, c(1600, 2400))
#
setwd(old_wd)

bpollner/flowdex documentation built on March 31, 2022, 3:21 a.m.