README.md

In cbg-ethz/WWdPCR: Analysis of Drop-Off Duplex dPCR Assays

WWdPCR

WWdPCR is a package to analyse dPCR duplex assays. The main use cases are: computing confidence intervals for the variant proprotion in a dPCR duplex assay, fitting a logistic growth model to the variant proportion with time series duplex assay dPCR data (also including background prevalence) to estimate growth rates. The logistic growth functions (that include background prevalence) can also be applied to binomial data.

If you use WWdPCR, please cite Caduff et al. 2021 (https://doi.org/10.1101/2021.08.22.21262024).

Installation

WWdPCR is not yet on CRAN.

You can install the development version from GitHub with:

# install.packages("devtools")
devtools::install_github("cbg-ethz/WWdPCR")

Examples

To see examples on how to use WWdPCR, compile the vignettes on installing:

# install.packages("devtools")
# install.packages("rmarkdown")
devtools::install_github("cbg-ethz/WWdPCR", build_vignettes=TRUE)

And then run the vignettes:

vignette("fitting_dPCR_data")
vignette("fitting_binomial_data")
vignette("example_confints")

cbg-ethz/WWdPCR documentation built on Jan. 23, 2022, 2:45 p.m.