HRGPcurves: Fluorescence curves for HRGP in transgenic roots under...

Description Usage Format Author(s) Examples

Description

Roots of a wildtype as well as transgenic roots are observed under standard nutritional conditions and under phosphorus deficiency. The relative expression of HRGP in with pK7GWIWG2(II)::HRGP generated roots are compared to the wildtype and to a standard gene AtUBC-2.

Usage

1

Format

A data frame with 3240 observations on the following 7 variables.

Well

a factor with well IDs

Content

factor levels denoting biological replicates

Target

2 different genes HRGP and At-UBC2 as control

P

+/- Phosphorus

WT

Wildtype yea/no

Cycle

cycle number

Fluorescence

observed fluorescence (proportional to PCR material)

Author(s)

Martin Hinrichs <martin_hinrichs@t-online.de>

Examples

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3
data(HRGPcurves)
ggplot(HRGPcurves, aes(x=Cycle, y=Fluorescence, colour=P:WT,
group=Well)) + geom_line() + facet_wrap(~Target)

daniel-gerhard/qpcrmix documentation built on May 14, 2019, 3:39 p.m.