pt3: PT3 expression at phosphate deficiency
In daniel-gerhard/qpcrmix: Mixed model analysis of qPCR data

Description Usage Format Author(s) Examples

RT-PCR analysis of the phosphate transporter PT3 in rice at several time periods of phosphate deficiency.

data(pt3)

A data frame with 90 observations on the following 5 variables.

Well: a factor with well IDs
Content: factor levels denoting biological replicates
Target: 2 different genes PT3 and eEf (control)
Time: 0,2,4,6, and 8 hours in a nutrient solution at low phosphate content
Ct: a numeric response variable with estimated threshold cycles

Melanie Bremer <bremer@pflern.uni-hannover.de>

data(pt3)
ptct <- qpcr(data=pt3, response="Ct",
             gene="Target", control_gene="eEf",
             fixed1="Time",
             rep_id="Content",
             block=FALSE, splitplot=FALSE,
             contrasts=TRUE, interaction=FALSE, adjusted=TRUE)

# model
ptct$model
# testing deltaCt contrasts
ptct$pvalues