IPE: IPE dataset

In daniel-gerhard/qpcrmix: Mixed model analysis of qPCR data

Description

Dataset from the Institute of plant nutrition (LUH). Gene XTH should be upregulated at phosphate deficiency.

Usage

data(IPE)

Format

A data frame with 72 observations on the following 5 variables.

Well

a factor with well IDs

Content

ID of biological replicates

Target

2 different genes: UBQ (control) and XTH (gene of interest)

Sample

treatment factor

Ct

a numeric response variable with estimated threshold cycles

Author(s)

Melanie Bremer <[e-mail]>

Examples

data(IPE)
qipe <- qpcr(data=IPE, response="Ct",
             gene="Target", control_gene="UBQ",
             fixed1="Sample",
             rep_id="Content",
             block=FALSE, splitplot=FALSE,
             contrasts=TRUE, interaction=FALSE, adjusted=TRUE)

# model
qipe$model
# testing deltaCt contrasts
qipe$pvalues 

daniel-gerhard/qpcrmix documentation built on May 14, 2019, 3:39 p.m.