scigenex: The scigenex package (Single-Cell Informative GENe Explorer)

cluster_set_from_seurat

R Documentation

Transform a Seurat objects / FindAllMarkers result into a ClusterSet.

Description

Transform a Seurat objects into a ClusterSet.

Usage

cluster_set_from_seurat(
  object = NULL,
  markers = NULL,
  which_slot = c("data", "counts", "sct")
)

Arguments

`object`	A Seurat object.
`markers`	A Seurat::FindAllMarkers() result or a named vector (clusters with gene_names as named).
`which_slot`	One of 'data', 'counts' or 'sct'. The slot to extract from the seurat object to perform clustering analysis. SCT is the recommended method from Seurat package when working with spatial transcriptomics data.

Examples

## From a scRNA-seq/Seurat object
library(SeuratObject)
library(Seurat)
data("pbmc_small", package="SeuratObject")
cs <- cluster_set_from_seurat(pbmc_small, Seurat::FindAllMarkers(pbmc_small))
plot_heatmap(cs)
plot_heatmap(cs)
plot_heatmap(cs[1,])
plot_heatmap(cs, cell_clusters = Seurat::Idents(pbmc_small))
plot_heatmap(cs[1,Idents(pbmc_small) == "0"], 
             cell_clusters = Seurat::Idents(pbmc_small), label_size = 6)
plot_profiles(cs, ident = Seurat::Idents(pbmc_small))

dputhier/scigenex documentation built on Dec. 20, 2024, 2:22 a.m.