gl.report.rdepth: Reports summary of Read Depth for each locus

In green-striped-gecko/dartR: Importing and Analysing 'SNP' and 'Silicodart' Data Generated by Genome-Wide Restriction Fragment Analysis

Reports summary of Read Depth for each locus

Description

SNP datasets generated by DArT report AvgCountRef and AvgCountSnp as counts of sequence tags for the reference and alternate alleles respectively. These can be used to back calculate Read Depth. Fragment presence/absence datasets as provided by DArT (SilicoDArT) provide Average Read Depth and Standard Deviation of Read Depth as standard columns in their report. This function reports the read depth by locus for each of several quantiles.

Usage

gl.report.rdepth(
  x,
  plot.out = TRUE,
  plot_theme = theme_dartR(),
  plot_colors = two_colors,
  save2tmp = FALSE,
  verbose = NULL
)

Arguments

x	Name of the genlight object containing the SNP or presence/absence (SilicoDArT) data [required].
plot.out	Specify if plot is to be produced [default TRUE].
plot_theme	Theme for the plot. See Details for options [default theme_dartR()].
plot_colors	List of two color names for the borders and fill of the plots [default two_colors].
save2tmp	If TRUE, saves any ggplots and listings to the session temporary directory (tempdir) [default FALSE].
verbose	Verbosity: 0, silent or fatal errors; 1, begin and end; 2, progress log; 3, progress and results summary; 5, full report [default 2, unless specified using gl.set.verbosity].

Details

The function displays a table of minimum, maximum, mean and quantiles for read depth against possible thresholds that might subsequently be specified in gl.filter.rdepth. If plot.out=TRUE, display also includes a boxplot and a histogram to guide in the selection of a threshold for filtering on read depth.

If save2tmp=TRUE, ggplots and relevant tabulations are saved to the session's temp directory (tempdir).

For examples of themes, see

• https://ggplot2.tidyverse.org/reference/ggtheme.html and

• https://yutannihilation.github.io/allYourFigureAreBelongToUs/ggthemes/

Value

An unaltered genlight object

Author(s)

Custodian: Arthur Georges – Post to https://groups.google.com/d/forum/dartr

See Also

gl.filter.rdepth

Other report functions: gl.report.bases(), gl.report.callrate(), gl.report.diversity(), gl.report.hamming(), gl.report.heterozygosity(), gl.report.hwe(), gl.report.ld.map(), gl.report.locmetric(), gl.report.maf(), gl.report.monomorphs(), gl.report.overshoot(), gl.report.pa(), gl.report.parent.offspring(), gl.report.reproducibility(), gl.report.secondaries(), gl.report.sexlinked(), gl.report.taglength()

Examples

 
# SNP data
df <- gl.report.rdepth(testset.gl)

df <- gl.report.rdepth(testset.gs)

green-striped-gecko/dartR documentation built on Sept. 7, 2024, 4:15 a.m.