Description Usage Arguments Details Value Author(s) See Also Examples

This function takes an expression matrix and true cell classes, and returns two square (JxJ) matrices, where J is the number of classes provided by true cell class.

1 | ```
createWeights(Y, classes)
``` |

`Y` |
The expression matrix. Rows are genes, columns are cells. |

`classes` |
True cell classes. |

There are two weight matrices W1 (score for putting two cells in the same cluster) and W0 (score for separating two cells in different clusters). We set W1 has diagonal values 1 and off-diagonal less than 1. To obtain off-diagonal values entry (i,j) in W1, we compute the mean expression profiles for all cell types from single-cell data, and then use the Pearson’s correlation of mean expression from cell types i and j as W1[i,j]. Note that in general, correlations among expression from different cell types are high because a majority of genes are not differentially expressed among cell types. To make W1 scores more distinctive, we take a marker selection step to pick the top 1000 genes with the largest variances of log expressions. The Pearson’s correlation of mean expression from these genes are then taken as the off-diagonal values for W1[i,j].

We make W0 has off diagonal values 1 and diagonal between 0 and 1, reflecting that keeping the separation existing in the reference receives full credit, but breaking a (weak) tie may not reduce the score completely to 0. We compute W0[i,i] based on the inter-cellular expression variances within cell type i. To be specific, we take expressions for all cells in cell type i and compute their Pearson’s correlations. W0[i,i] is defined as 1 minus the average inter-cellular Pearson’s correlations from all cells. Thus, for a tight cluster where the within cell type correlation is high, the score will be closer to 0, indicating stronger penalty for separating cells for such a cluster. On the other hand, for a loose cluster where the within cell type correlation is low, the score will be larger, indicating weaker penalty.

A list with following fields:

`W0` |
A square matrix of dimension JxJ, where J is the number of classes provided by true cell class. The (i,j)-th entry is the score of separating two cells of types i and j in different clusters. |

`W1` |
A square matrix of dimension JxJ, where J is the number of classes provided by by true cell class The(i,j)-th entry is the score for putting two cells of different types in the same cluster. |

Zhijin Wu <zhijin_wu@brown.edu>, Hao Wu <hao.wu@emory.edu>

wRI

1 2 | ```
data(Zhengmix8eq)
weights = createWeights(Y, trueclass)
``` |

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