ezcyclone: Cell cycle phase classification

View source: R/ezcyclone.R

ezcycloneR Documentation

Cell cycle phase classification

Description

Cell cycle phase classification using scran cyclone.

Usage

ezcyclone(
  sce,
  organism = c("hsa", "mmu"),
  gene.names = NULL,
  pairs = NULL,
  ncores = 1,
  seed = 100,
  iter = 1000,
  min.iter = 100,
  min.pairs = 50,
  verbose = TRUE
)

Arguments

sce

A SingleCellExperiment object containing expression values, usually counts.

organism

hsa or mmu for human or mouse genes pre-trained marker sets.

gene.names

Ensembl gene IDs for genes in sce. length(gene.names) should match with nrow(sce).

pairs

Pair information for training marker sets. If set to NULL, uses pre-trained marker sets. Some genes in pairs should overlap with row.names(sce).

ncores

Number of cores.

seed

Random seed.

iter

An integer scalar specifying the number of iterations for random sampling to obtain a cycle score.

min.iter

An integer scalar specifying the minimum number of iterations for score estimation.

min.pairs

An integer scalar specifying the minimum number of pairs for cycle estimation.

verbose

TRUE/FASLE for specifying whether diagnostics should be printed to screen.

Value

A list containing phases, scores, and normalized.scores.


huipan1973/ezscrnaseq documentation built on July 12, 2022, 9:36 p.m.