Description Usage Arguments Value Examples
A function that updates marker names in the files output by the preprocessing.batch function.
1 | nameUpdator(oldNames, newNames, files)
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oldNames |
A vector of marker names you wish to change |
newNames |
A vector of marker names you wish each oldNames to be changed to. |
files |
A list of "processed_sample_summary.csv" files output by the preprocessing.batch function, in which the name change will occur. |
Null
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 | #get meta-data
fn=system.file("extdata","fcs_info.csv",package="MetaCyto")
fcs_info=read.csv(fn,stringsAsFactors=FALSE,check.names=FALSE)
fcs_info$fcs_files=system.file("extdata",fcs_info$fcs_files,
package="MetaCyto")
# make sure the transformation parameter "b" and the "assay" argument
# are correct of FCM and CyTOF files
b=assay=rep(NA,nrow(fcs_info))
b[grepl("CyTOF",fcs_info$study_id)]=1/8
b[grepl("FCM",fcs_info$study_id)]=1/150
assay[grepl("CyTOF",fcs_info$study_id)]="CyTOF"
assay[grepl("FCM",fcs_info$study_id)]="FCM"
# preprocessing
preprocessing.batch(inputMeta=fcs_info,
assay=assay,
b=b,
outpath="Example_Result/preprocess_output",
excludeTransformParameters=c("FSC-A","FSC-W","FSC-H",
"Time","Cell_length"))
# Make sure marker names are consistant in different studies
files=list.files("Example_Result",pattern="processed_sample",recursive=TRUE,
full.names=TRUE)
nameUpdator("CD8B","CD8",files)
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