Man pages for ipb-halle/GoldenMutagenesis
A tool to calculate and validate primers for Golden Gate Cloning
| base_distribution | Create a graphical evaluation of sequencing results |
| calculate_tm_nnb | Calculate melting temperature based on next neighbor... |
| cuf | The Codon Usage Table which is being used to select the codon... |
| domesticate | Domestication of the input sequence |
| get_cu_table | Select a Codon Usage Table |
| input_sequence | The sequence which should be modified. This is an object of... |
| list_cu_table | List the available Codon Usage Tables |
| MSD_BsaI_result_lv2 | Designed primers for Multiple Site Directed Mutagenesis |
| MSD_BsaI_setup_lv2 | Basic setup for Multiple Site Directed Mutagenesis |
| mutate_msd | Calculate Primers for Multiple Site Saturation Mutagenesis |
| mutate_spm | Calculate Primers for Point Mutagenesis |
| mutations | The desired mutations |
| order_replacements | Order the replacement list |
| Point_Mutagenesis_BbsI_result | Designed primers for Point Mutagenesis |
| Point_Mutagenesis_BbsI_setup | Basic setup for Point Mutagenesis |
| primer_add_level | Add a level to exisiting Primerset |
| primer_prepare_level | Prepare a Single Point Mutation Primerset to be used in Level... |
| primers | Generated Primers From Multiple Site Directed Mutagenesis or... |
| print_primer-methods | A function to generate an output text for primers |
| recognition_site_bbsi | The BbsI recognition site |
| recognition_site_bsai | The BsaI recognition site |
