calculate_tm_nnb: Calculate melting temperature based on next neighbor...

In ipb-halle/GoldenMutagenesis: A tool to calculate and validate primers for Golden Gate Cloning

Description

The implementation is based on the explanations of http://biotools.nubic.northwestern.edu/OligoCalc.html.

Usage

calculate_tm_nnb(
  oligo_sequence,
  primer_concentration = 50,
  salt_concentration = 50,
  offset = 0
)

Arguments

oligo_sequence	A string containing an oligo sequence.
primer_concentration	The concentration of the primer in nanomole [default: 50]
salt_concentration	The concentration of Na+ in nanomole [default: 50]
offset	You can skip a prefix of your oligo sequence with this parameter. The first n bases are not considered in the calculation. [default: 0]

Details

More details at https://doi.org/10.1093/nar/gkm234

Value

An array or a list with values for the codons/amino acids.

The melting temperature in print('\u00B0')C

Examples

## Not run: 
GoldenMutagenesis::calculate_tm_nnb("AAAAAATGGTGTGTGATGTGTCCCTCTATC")

## End(Not run)

ipb-halle/GoldenMutagenesis documentation built on Sept. 1, 2020, 3:24 p.m.