library(knitr) ## use pngquant to reduce size of PNG images knit_hooks$set(pngquant = hook_pngquant) pngquant <- "--speed=1 --quality=0-25" # in case pngquant isn't available if (!nzchar(Sys.which("pngquant"))) pngquant <- NULL
options(width = 80)
nH2O <- "<i>n</i><sub>H<sub>2</sub>O</sub>" nO2 <- "<i>n</i><sub>O<sub>2</sub></sub>"
This vignette runs the code to make the plots from the following paper:
Dick JM, Kang X. 2023. chem16S: community-level chemical metrics for exploring genomic adaptation to environments. Bioinformatics 39(9): btad564. doi: 10.1093/bioinformatics/btad564
Update (2024-11-21): The figure has been updated to use GTDB release 220 for reference proteomes and taxonomic classification of the mouse dataset.
This vignette was compiled on r Sys.Date()
with
JMDplots r packageDescription("JMDplots")$Version
and
chem16S r packageDescription("chem16S")$Version
.
library(JMDplots)
chem16S_1()
Data sources:
The GlobalPatterns
dataset is from the phyloseq package [@MH13] and is adapted from @CLW+11.
The mouse gut dataset was created by analyzing sequence data from @SSZ+12 (downloaded here and used in the mothur MiSeq SOP and DADA2 Pipeline Tutorial) with the DADA2 pipeline modified to use 16S rRNA sequences from GTDB release 220 [@Ali24] for taxonomic classifications.
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