relex: Calculate The 2^dCt

In jrijn/Rsome: Analyse RT-qPCR Data

Description

This function calculates the relative expression from a cqimport object. Furthermore, it creates separate .csv files of the relative expression summary, the samples which don't meet the set criteria, and the -RT values.

Usage

relex(tablename, household = "Gapdh", SDcutoff = 1, Cqcutoff = 35)

Arguments

tablename:	a cqimport object
household:	defaults to "Gapdh". Accepts a string to define the household gene.
SDcutoff:	Defines the cutoff value for the standard deviation of the mean Cq. Default = 1.
Cqcutoff:	Defines the cutoff value for the mean Cq. Default = 35.

Examples

df <- cqimport(filename)
rel.expression <- relex(df, household = "Gapdh")

jrijn/Rsome documentation built on April 13, 2020, 7:52 p.m.