pacific_oyster_18S_V4: pacific_oyster_18S_V4:

In kevinzhongxu/CasOligo: A R package to search the gRNA-target-site oligo for designing the taxon-specific guide RNA used for CRISPR-Cas Selective Amplicon Sequencing (CCSAS) to assess the eukaryotic microbiome of hosts (e.g. metazoans, plant)

pacific_oyster_18S_V4:

Description

18S rRNA gene sequence of pacific oyster at V4 region that amplified by primers "TAReuk454FWD1 and TAReukREV3" (Stoeck et al., 2010). This serves as an example of input fasta file for cas9.gRNA.oligo1, cas9.gRNA.oligo2, cas12a.gRNA.oligo1 and cas12a.gRNA.oligo2.

Usage

data(pacific_oyster_18S_V4)

Format

A data frame

References

Stoeck T, Bass D, Nebel M, Christen R, Jones MD, Breiner HW, Richards TA. (2010) Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water Mol. Ecol. 19, 21–31. (PubMed)

Examples

data(pacific_oyster_18S_V4)

kevinzhongxu/CasOligo documentation built on Feb. 25, 2025, 8:43 a.m.