prb.score: prb.score:

In kevinzhongxu/CasOligo: A R package to search the gRNA-target-site oligo for designing the taxon-specific guide RNA used for CRISPR-Cas Selective Amplicon Sequencing (CCSAS) to assess the eukaryotic microbiome of hosts (e.g. metazoans, plant)

prb.score:

Description

Data for the database of gRNA-target-site oligos that are CRISPR-Cas9 compatible, and which is specific to target the V4 region of 18S rRNA gene amplified by primers "TAReuk454FWD1 and TAReukREV3" (Stoeck et al., 2010).

Usage

data(prb.score)

Format

A data frame

References

Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO (2013) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Nucl. Acids Res. 41 (D1): D590-D596. (PubMed)

Stoeck T, Bass D, Nebel M, Christen R, Jones MD, Breiner HW, Richards TA. (2010) Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water Mol. Ecol. 19, 21–31. (PubMed)

Examples

data(prb.score)

kevinzhongxu/CasOligo documentation built on Feb. 25, 2025, 8:43 a.m.