silva_v119_18S_99rep_V4: silva_v119_18S_99rep_V4:

In kevinzhongxu/CasOligo: A R package to search the gRNA-target-site oligo for designing the taxon-specific guide RNA used for CRISPR-Cas Selective Amplicon Sequencing (CCSAS) to assess the eukaryotic microbiome of hosts (e.g. metazoans, plant)

silva_v119_18S_99rep_V4:

Description

Reference database of the 18S rRNA gene at V4 region amplified by primers TAReuk454FWD1 and TAReukREV3 (Stoeck et al., 2010), which uses for the identify the gRNA-target-sequence oligos.

Usage

data(silva_v119_18S_99rep_V4)

Format

A DNAString fasta input

References

Stoeck T, Bass D, Nebel M, Christen R, Jones MD, Breiner HW, Richards TA. (2010) Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water Mol. Ecol. 19, 21–31. (PubMed)

Examples

data(silva_v119_18S_99rep_V4)

kevinzhongxu/CasOligo documentation built on Feb. 25, 2025, 8:43 a.m.