NEWS.md

In mikelove/tximeta: Transcript Quantification Import with Automatic Metadata

tximeta 1.21.4

• Changing language in docs to "digest" instead of "checksum".

tximeta 1.21.3

• GENCODE 44 (H.s.), M34 (M.m), and Ensembl 111
• RefSeq p13 for human, p6 for mouse

tximeta 1.20.0

• Add argument to summarizeToGene(): assignRanges that takes either "range" (default) or "abundant", and determines the ranges that are attached to the SE (rowRanges). Note that this new argument does not affect the data aggregation at all (counts and abundance are summarized to gene by tximport). The default behavior of summarizeToGene() returns ranges that correspond to the range of the isoforms of the gene, that is the leftmost basepair to the rightmost basepair of any isoform. The non-default "abundant" instead returns the range of the most abundant isoform in the data, averaging over samples. Information about the choice of range is included in mcols
• Added support for piscem-infer: type="piscem" also auto-detected from file ending.

tximeta 1.19.8

• Added support for piscem-infer: type="piscem" also auto-detected from file ending.

tximeta 1.19.6

• Fixed genome build for mouse M26 and higher to GRCm39, thanks to Charlotte Soneson.

tximeta 1.14.0

• Allow GTF specification in linkedTxome to be a serialized GRanges file (a file path to a .rda or .RData file). This bypasses some apparent issue where makeTxDbFromGFF fails while makeTxDbFromGRanges works.
• Up to GENCODE 40 (H.s.), M29 (M.m), and Ensembl 106

tximeta 1.10.0

• Added more tximeta() messaging about specifying the 'source' in linkedTxome. Essentially, this triggers GTF processing behavior that users may want to avoid, and so specifying a string other than "Ensembl" may be preferred. Also added note to vignette.
• Added note to vignette about alevin import with tximeta where the 'tgMap' step requires gene IDs and not gene symbols.
• Added hashes for: GENCODE 38 (H.s.), M27 (M.m), and Ensembl 104; GENCODE 37 (H.s.), M26 (M.m), and Ensembl 103; GENCODE 36 and Ensembl 102.
• Fixed a bug around AnnotationHub pulldown when using RefSeq as the source.
• Fixed a bug where multiple parsed Ensembl GTF TxDb would be added to the BiocFileCache with the same rname.

tximeta 1.8.0

• Added 'fromDb' argument to addIds() to allow IDs to be added from the associated TxDb/EnsDb instead of the org package (which is used by default). Feature suggestion from Kristoffer Vitting-Seerup.
• Added function retrieveCDNA() that will download or load a cached version of the transcript sequences used for quantification. Note that the returned sequences are not ordered or matched to the rows of the SummarizedExperiment object. Feature suggestion from Kristoffer Vitting-Seerup.
• Added function addCDS() that will add CDS ranges for coding transcripts (and fills in original ranges for non-coding), as well as a 'coding' column as a logical indicator. Feature suggestion from Kristoffer Vitting-Seerup.
• Added option that environmental variable TXIMETA_HUB_CACHE can be used to set tximeta's cache location, to avoid prompting the user on the first run of tximeta().
• tximeta() will now pull GENCODE TxDb from AnnotationHub when it is listed there (only Homo sapiens are at this point in time). Thanks to Leonardo Collado-Torres for the suggestion!
• Now summarizeToGene() will add a column tx_ids, which is a CharacterList of the transcript IDs.

tximeta 1.6.0

• Added PLOS Computational Biology citation! :-)
• Added function splitSE to split one assay of a SummarizedExperiment into multiple assays, each containing features of a given type.
• Added a wrapper function makeDGEList() to simplify making a DGEList for use with edgeR. See vignette for example.
• tximeta will now make use of EnsDb created and distributed on AnnotationHub, unless useHub=FALSE. Also, a new function retrieveDb() can be called on a SummarizedExperiment to retrieve the underlying TxDb or EnsDb.
• tximeta can now use customMetaInfo argument to locate a custom metadata information file such as meta_info.json, which should contain a tage, index_seq_hash, with the SHA-256 hash value of the reference transcripts.
• Added markDuplicateTxps argument to add hasDuplicate and duplicates columns to rowData of SummarizedExperiment. One note is that, for efficiency, this argument and cleanDuplicateTxps will now share a duplicates CharacterList that is stored in the BiocFileCache, with the name dups-.... Therefore, if you have previously used cleanDuplicateTxps, you may need to bfcremove() any dups-... entries. Summarization to gene level will keep track of numDupSets per gene which informs about the number of transcripts sets (equivalence classes by transcript sequence content).
• If during the indexing step, user didn't use --gencode for a Gencode transcriptome file, tximeta will deal with this internally now by stripping all characters after the vertical bar |, in order to match long transcript names in the quant.sf files to the correct transcript names in the GTF.

tximeta 1.4.0

• tximeta will now pull down RefSeq seqinfo, using the dirname() of the GTF location, and assuming some consistency in the structure of the assembly_report.txt that is located in the same directory. Needs more testing though across releases and organisms.
• expanded caching of ranges to exons and genes as well. Exons in particular take a long time to build from TxDb, so this saves quite a lot of time.
• new 'addExons' function will add exons to trancript-level summarized experiments, by replacing transcript GRanges with exon-by-transcript GRangesList. Purposely designed only for transcript-level, see note in ?addExons
• tximeta now also caches the transcript ranges themselves, rather than just the TxDb. This shaves extra seconds off the tximeta() call!
• add 'skipSeqinfo' argument, which avoids attempting to fetch chromosome information (from UCSC) if set to TRUE.

tximeta 1.2.0

• Specifying gene=TRUE in addIds() when rows are transcripts will attempt to use a gene_id column to map the IDs. This usually gives a better mapping rate.
• Cut off version number from Ensembl names only (not GENCODE)
• Added 'cleanDuplicateTxps' argument, which does a lot of work for the user: it downloads the FASTA from the source, identifies duplicate transcripts (identical cDNA sequence) then looks to see if transcripts that are in the quantification files, but missing from the GTF, could be renamed from the list of duplicate transcripts such that they would be present in the GTF.
• Added coding * non-coding combinations of Ensembl transcriptomes to the hash table. Must be in this order: coding, then non-coding.
• Added support for dammit de novo transcriptomes.
• Added summarizeToGene as a method, to avoid conflicts with tximport.
• Added in Charlotte's code to split out GENCODE and Ensembl code for generating transcript ranges.

mikelove/tximeta documentation built on Feb. 26, 2024, 4:32 a.m.