FeaturePlotly: FeaturePlotly

Description Usage Arguments Value

Description

Create a scatterplot of a given dimensional reduction set for a Seurat object, coloring and sizing points by the expression level of the chosen feature. Requrires a Seurat object with the reduction to be used in the corresponding object@dr slot

Usage

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FeaturePlotly(object, feature = NULL, reduction = "umap",
  assay = NULL, slot = NULL, filter_zeros = TRUE, dim_1 = 1,
  dim_2 = 2, pt_scale = 5, pt_shape = "circle", opacity = 1,
  colors_use = "Red", reverse_color_scale = FALSE, bins = 10,
  plot_height = 900, plot_width = 900, plot_title = FALSE,
  pt_info = NULL, legend = TRUE, legend_font_size = 12,
  return = FALSE)

Arguments

object

Seurat object

feature

Feature values to display. Works with anything Seurat::FetchData can retrieve.

reduction

Dimensional reduction to display. Default: umap

assay

Assay to pull values from for feature. Default: NULL

slot

Slot to pull values from for feature. Default: NULL

filter_zeros

Remove points with no expression data. Default: TRUE

dim_1

Dimension to display on the x-axis. Default: 1

dim_2

Dimension to display on the y-axis. Default: 2

pt_scale

Factor by which to multiply the size of the points. Default: 5

pt_shape

Shape to use for the points. Default = circle

opacity

Transparency level to use for the points, on a 0-1 scale. Default: 1

colors_use

Color palette to use. Default: Reds

reverse_color_scale

Reverse the color scale. Default = FALSE

bins

Number of bins to use in dividing expression levels.. Default: 10

plot_height

Plot height in pixels. Default: 900

plot_width

Plot width in pixels. Default: 900

plot_title

Display title with the name of the feature?. Default TRUE

pt_info

Meta.data columns to add to the hoverinfo popup. Default: ident

legend

Display legend?. Default: TRUE

legend_font_size

Legend font size. Default: 12

return

Return the plot dataframe instead of displaying it. Default: FALSE

Value

plotly object


milescsmith/SeuratPlotly documentation built on June 5, 2019, 1:16 a.m.