# load data
data(liver.toxicity)
# run tuning
tune <- tune.pca(liver.toxicity$gene, center = TRUE, scale = TRUE)
plot(tune)
# set up multilevel dataset
repeat.indiv <- c(1, 2, 1, 2, 1, 2, 1, 2, 3, 3, 4, 3, 4, 3, 4, 4, 5, 6, 5, 5,
6, 5, 6, 7, 7, 8, 6, 7, 8, 7, 8, 8, 9, 10, 9, 10, 11, 9, 9,
10, 11, 12, 12, 10, 11, 12, 11, 12, 13, 14, 13, 14, 13, 14,
13, 14, 15, 16, 15, 16, 15, 16, 15, 16)
design <- data.frame(sample = repeat.indiv)
# run tuning
tune <- tune.pca(liver.toxicity$gene, center = TRUE, scale = TRUE, multilevel = design)
plot(tune)
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