#!/usr/bin/env Rscript
#
# Copyright 2013 - 2022 Michael K. Schuster
#
# Biomedical Sequencing Facility (BSF), part of the genomics core facility of
# the Research Center for Molecular Medicine (CeMM) of the Austrian Academy of
# Sciences and the Medical University of Vienna (MUW).
#
#
# This file is part of BSF R.
#
# BSF R is free software: you can redistribute it and/or modify
# it under the terms of the GNU Lesser General Public License as published by
# the Free Software Foundation, either version 3 of the License, or
# (at your option) any later version.
#
# BSF R is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
# GNU Lesser General Public License for more details.
#
# You should have received a copy of the GNU Lesser General Public License
# along with BSF R. If not, see <http://www.gnu.org/licenses/>.
# Description -------------------------------------------------------------
# BSF R Script to merge two GenomeInfoDb::Seqinfo objects.
#
# Output files:
# - <output_directory>/<genome_version>_seqinfo.rds
# Option Parsing ----------------------------------------------------------
suppressPackageStartupMessages(expr = library(package = "optparse"))
argument_list <-
optparse::parse_args(object = optparse::OptionParser(
option_list = list(
optparse::make_option(
opt_str = "--verbose",
action = "store_true",
default = TRUE,
help = "Print extra output [default]",
type = "logical"
),
optparse::make_option(
opt_str = "--quiet",
action = "store_false",
default = FALSE,
dest = "verbose",
help = "Print little output",
type = "logical"
),
optparse::make_option(
opt_str = "--seqinfo-path-1",
dest = "seqinfo_path_1",
help = "Seqinfo file path (genome_seqinfo.rds)",
type = "character"
),
optparse::make_option(
opt_str = "--seqinfo-path-2",
dest = "seqinfo_path_2",
help = "Seqinfo file path (genome_seqinfo.rds)",
type = "character"
),
optparse::make_option(
opt_str = "--genome-version",
dest = "genome_version",
help = "Genome assembly version",
type = "character"
),
optparse::make_option(
opt_str = "--output-directory",
default = ".",
dest = "output_directory",
help = "Output directory path [.]",
type = "character"
)
)
))
if (is.null(x = argument_list$genome_version)) {
stop("Missing --genome-version option")
}
if (is.null(x = argument_list$seqinfo_path_1)) {
stop("Missing --seqinfo-path-1 option")
}
if (is.null(x = argument_list$seqinfo_path_2)) {
stop("Missing --seqinfo-path-2 option")
}
# Library Import ----------------------------------------------------------
# CRAN r-lib
suppressPackageStartupMessages(expr = library(package = "sessioninfo"))
# CRAN Tidyverse
suppressPackageStartupMessages(expr = library(package = "readr"))
# Bioconductor
suppressPackageStartupMessages(expr = library(package = "BiocVersion"))
suppressPackageStartupMessages(expr = library(package = "GenomeInfoDb"))
seqinfo_1 <- readr::read_rds(file = argument_list$seqinfo_path_1)
seqinfo_2 <- readr::read_rds(file = argument_list$seqinfo_path_2)
seqinfo_object <- GenomeInfoDb::merge(x = seqinfo_1, y = seqinfo_2)
readr::write_rds(
x = seqinfo_object,
file = file.path(argument_list$output_directory,
paste(
paste(argument_list$genome_version, "seqinfo", sep = "_"),
"rds",
sep = "."
)),
compress = "gz"
)
rm(seqinfo_object, seqinfo_2, seqinfo_1, argument_list)
message("All done")
# Finally, print all objects that have not been removed from the environment.
if (length(x = ls())) {
print(x = "Remaining objects:")
print(x = ls())
}
print(x = sessioninfo::session_info())
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.