flux_measurements: Measurements for extracellular flux calculations
In oldhamlab/Copeland.2021.hypoxia.flux: What the Package Does (One Line, Title Case)

A data set containing the interpolated metabolite concentrations, cell counts, and evaporation volumes for extracellular flux determinations.

1	flux_measurements

An object of class tbl_df (inherits from tbl, data.frame) with 24126 rows and 15 columns.

cell_type: lf = lung fibroblasts
pasmc = pulmonary artery smooth muscle cells
experiment: 02 = 0.2% oxygen for hypoxia
05 = 0.5% oxygen for hypoxia
bay = molidustat treatment
05-bay = 0.5% oxygen plus molidustat
batch: a group of biological replicates analyzed similarly
date: start date of an experiment
metabolite: name of measurement
abbreviation: abbreviated metabolite name
detector: fld = HPLC fluorescence detector
mwd = HPLC multi-wavelength detector
enzyme = enzymatic assay
hplc = OPD-derivatized HPLC detection
lcms = liquid chromatography-mass spectrometry assay
picogreen = fluorescence dye labeling of DNA
type: cells = conditioned medium
empty = unconditioned medium
oxygen: ambient oxygen level
treatment: None = no treatment
DMSO = 0.1% DMSO
BAY = 10 μM molidustat
time: hours
well: denotes technical replicates in each experiment
conc: measured in number for cells and μM for metabolites
volume: measured in mL, extrapolated from evaporation controls
nmol: metabolite mass

oldhamlab/Copeland.2021.hypoxia.flux documentation built on Feb. 5, 2022, 8:31 p.m.

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