boeal: Number of nalidixic acid-resistant mutants from Boe et al....
In rcqls/flanRcpp: Fluctuation analysis of mutant cells

Description Usage Format Source Examples

Samples from Table 4 p. 2784-2785 of Boe and al (1994).

Plates with more than 512 mutants could not be counted with precision, hence the value 512 must be understood as “512 or more”.

1	data(boeal)

A list of 23 samples of mutants counts, each named "B<index of the sample>".

The i-th sample of the list includes the i-th column of the table.

L. Boe, T. Tolker-Nielsen, K. M. Eegholm, H. Spliid, and A. Vrang: Fluctuation analysis of mutations to nalidixic acid resistance in Escherichia Coli, J. Bacteriol., 176(10):2781-2787 (1994)

b <- unlist(boeal)              # concatenate all samples
ml <- mutestim(b)               # maximum likelihood
gf <- mutestim(b,method="GF")   # generating function
p0 <- mutestim(b,method="P0")   # P0 method
cbind(ml,gf,p0)                 # compare 3 methods

                                # test values of mutations and fitness
flan.test(b,alternative=c("greater","less"),mutations0=0.6,fitness0=1)

b1 <- unlist(boeal[1:10])       # first 10 samples
b2 <- unlist(boeal[11:20])      # next 10 samples
flan.test(list(b1,b2))          # test equality