pqn: Perform Probabilistic Quotient Normalization

In ricoderks/Rcpm: General functions for the Center of Proteomics and Metabolomics (LUMC)

Perform Probabilistic Quotient Normalization

Description

Perform Probabilistic Quotient Normalization

Usage

pqn(X, n = "median", QC = NULL)

Arguments

X	matrix to normalize samples * variables (rows * columns)
n	normalization reference: "mean" for using the overall average of variables as reference or "median" (default) for using the overall median of variables as reference
QC	vector of number(s) to specify samples which average to use as reference (e.g. QC samples)

Details

First a total area normalization should be done before PQN is applied.

Value

Normalized table samples * variables (rows * columns)

Author(s)

E. Nevedomskaya

Rico Derks

References

Dieterle, F., Ross, A., Schlotterbeck, G. & Senn, H. Probabilistic Quotient Normalization as Robust Method to Account for Dilution of Complex Biological Mixtures. Application in H1 NMR Metabonomics. Anal. Chem. 78, 4281-4290 (2006).

ricoderks/Rcpm documentation built on May 18, 2022, 7:49 a.m.