get_positive_antigens: Returns positive antigens based on low control value and a...
In rmsharp/snprcspf: SNPRC SPF Colony Screening Data Software Tool Package

From e-mail 2015-09-22 13:32 by Luis Giavedoni

1	get_positive_antigens(d_mfi_df, mult = 1)

`d_mfi_df`	dataframe wih standardized antigen reactivity measures
`mult`	multiplier used to adjust cutoff level.

Mark,

When the CNT LOW is below 2.5, we use the CNT LOW score to assign positive reactivity. Any score equal or higher than the CNT LOW will be scored as positive.

When the CNT LOW is above 2.5, we use 2.5 as the cut off. Any score equal or higher than 2.5 will be scored as positive.

I know that I said that the low score was 3.0, but after review of current Charles River documents, the recommendation is for the score to be 2.5.

Regards,

Luis

Thus, the function used is df$result <- ifelse(df$val >= min(2.5, df$cutoff), "P", "N")

SPF Colony Testing Plate 96 06-09-16.xlsx has an extra low control ICL CNT LOW (dup). Controls with stri_detect_fixed(tolower(name), "(dup)") == TRUE are ignored.

rmsharp/snprcspf documentation built on June 15, 2020, 11:21 a.m.