An example FCS flowSet.
This is an object of class flowSet composed of 7 flowFrames.
The flowSet corresponds to a Leukemia/Lymphoma panel for a sample. Each flowFrame correponds to one tube. The first one is an isotype control, and the rest are stained with different antibody cocktails. Parameter 5 (FL3 Log) is notable in that it is CD45-ECD for all of the tubes. This makes it possible to gate the entire collection of tubes using CD45 vs SSC. This set has a very similar distribution of CD45 vs SSC across all 7 tubes.
column names: FS Lin SS Log FL1 Log FL2 Log FL3 Log FL4 Log FL5 Log
Data were generously provided by Clarient, Inc.
W.T. Rogers and H. A. Holyst, “flowFP: A Bioconductor Package for Fingerprinting Flow Cytometric Data”, Submitted to: Advances in Bioinformatics, April 1, 2009.
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