calibrate_ssc_conversion: Calibrate Detector Response Curve

In rogerswt/flowMie: Modeling a Detector Response to Scattering of (Multi-layer) Spherical Particles

Description

This function uses the signal from a calibration bead to determine the gain factor for the instrument. This factor converts the output of calculate_detector_response such that it reads out in the same units as reported by the detector.

This function is a precursor to the use of the mie_transform function, which calculates vesicle size and appends it to a flowFrame.

Usage

calibrate_ssc_conversion(
  detector = create_detector(),
  bead = create_SI(d = 200),
  bead_mfi = 60000,
  show = FALSE
)

Arguments

detector	A description of the detector. Default = create_detector().
bead	A description of the calibration bead used. Default is a silica bead with a diameter of 200nm.
bead_mfi	The "MFI" of the signal generated by the calibration bead. Default = 60000.
show	Display a graph of detector response as a function of EV diameter. Default = FALSE.

Value

A list, with elements:

gain

The deduced gain factor for the detector

lut

A lookup table relating EV size to the corresponding detector signal

rogerswt/flowMie documentation built on Jan. 31, 2021, 8 a.m.