mie_transform: Compute the Mie Transform

In rogerswt/flowMie: Modeling a Detector Response to Scattering of (Multi-layer) Spherical Particles

Description

Calculate the Mie Transform, which converts a scattering signal to a corresponding EV diameter by means of calculated scattering from a calibration particle.

Usage

mie_transform(
  ff,
  ssc_param = "SSC-A",
  transformation = c("none", "biexp"),
  detector
)

Arguments

ff	A flowFrame containing EV data.
ssc_param	The name of the scattering parameter to use for sizing. **NOTE: should be the same parameter used to detect the calibration bead in calibrate_ssc_conversion().**
transformation	What transformation was used on the scattering parameter. Ones currently supported include c("none", "biexp").
detector	A detector, having been calibrated by calibrate_ssc_conversion().

Details

There are several limitations of this function having to do with whether the scattering data have been transformed or not. Since the Mie Transform operates in linear space, any non-linear transformation must be inverted prior to calculating the Mie Transform. Currently, the only supported non-linear transformation is link[wadeTools]biexp.

Value

A flowFrame, with a new parameter called "Size (nm)".

rogerswt/flowMie documentation built on Jan. 31, 2021, 8 a.m.