In rosscm/fedup: Fisher's Test for Enrichment and Depletion of User-Defined Pathways
knitr::opts_chunk$set(
collapse = TRUE,
comment = "#>",
fig.path = "inst/figures/",
out.width = "100%"
)
**F**isher's Test for **E**nrichment and **D**epletion of **U**ser-Defined **P**athways
[](https://ci.appveyor.com/project/rosscm/fedup)
[](https://codecov.io/gh/rosscm/fedup)
`fedup` is an R package that tests for enrichment and depletion of user-defined
pathways using a Fisher's exact test. The method is designed for versatile
pathway annotation formats (eg. gmt, txt, xlsx) to allow the user to run
pathway analysis on custom annotations. This package is also
integrated with Cytoscape to provide network-based pathway visualization
that enhances the interpretability of the results.
This README will quickly demonstrate how to use `fedup` when testing two
sets of genes. Refer to full
[vignettes](https://www.bioconductor.org/packages/release/bioc/html/fedup.html)
for additional information and implementations (e.g., using single or
multiple test sets).
# Contents
- [System prerequisites](#system-prerequisites)
- [Installation](#installation)
- [Running the package](#running-the-package)
* [Input data](#input-data)
* [Pathway analysis](#pathway-analysis)
* [Dot plot](#dot-plot)
* [Enrichment map](#enrichment-map)
- [Versioning](#versioning)
- [Shoutouts](#shoutouts)
# System prerequisites
**R version** ≥ 4.1
**R packages**:
- **CRAN**: openxlsx, tibble, dplyr, data.table, ggplot2, ggthemes,
forcats, RColorBrewer
- **Bioconductor**: RCy3
# Installation
Install `fedup` from Bioconductor:
wzxhzdk:1
Or install the development version from Github:
wzxhzdk:2
Load necessary packages:
wzxhzdk:3
# Running the package
## Input data
Load test genes (`geneDouble`) and pathway annotations (`pathwaysGMT`):
wzxhzdk:4
Take a look at the data structure:
wzxhzdk:5
To see more info on this data, run `?geneDouble` or `?pathwaysGMT`.
You could also run `example("prepInput", package = "fedup")` or
`example("readPathways", package = "fedup")` to see exactly how the
data was generated using the `prepInput()` and `readPathways()` functions.
`?` and `example()` can be used on any other functions mentioned here to
see their documentation and run examples.
## Pathway analysis
Now use `runFedup` on the sample data:
wzxhzdk:6
The `fedupRes` output is a list of length `length(which(names(geneDouble) !=
"background"))`, corresponding to the number of test sets in `geneDouble`
(i.e., 2).
View `fedup` results for `FASN_negative` sorted by pvalue:
wzxhzdk:7
Let's also view `fedup` results for `FASN_positive`, sorted by pvalue:
wzxhzdk:8
## Dot plot
Prepare data for plotting via `dplyr` and `tidyr`:
wzxhzdk:9
Plot significant results (qvalue < 0.05) in the form of a dot plot via
`plotDotPlot`. Colour and facet the points by the `sign` column:
wzxhzdk:10
Look at all those chick... enrichments! This is a bit overwhelming, isn't it?
How do we interpret these 156 fairly redundant pathways in a way that doesn't
hurt our tired brains even more? Oh I know, let's use an enrichment map!
## Enrichment map
First, make sure to have
[Cytoscape](https://cytoscape.org/download.html) downloaded and and open
on your computer. You’ll also need to install the
[EnrichmentMap](http://apps.cytoscape.org/apps/enrichmentmap) (≥ v3.3.0) and
[AutoAnnotate](http://apps.cytoscape.org/apps/autoannotate) apps.
Then format results for compatibility with EnrichmentMap using `writeFemap`:
wzxhzdk:11
Prepare a pathway annotation file (gmt format) from the pathway list you
passed to `runFedup` using the `writePathways` function (you don't need to run
this function if your pathway annotations are already in gmt format, but it
doesn't hurt to make sure):
wzxhzdk:12
Cytoscape is open right? If so, run these lines and let the `plotFemap`
magic happen:
wzxhzdk:13
To note here, the EM nodes were coloured manually (by the same colours passed to
`plotDotPlot`) in Cytoscape via the *Change Colors* option in the EM panel.
A feature for automated dataset colouring is set to be released in
[version 3.3.2](https://github.com/BaderLab/EnrichmentMapApp/issues/455)
of EnrichmentMap.
This has effectively summarized the 156 pathways from our dot plot into 21
unique biological themes (including 4 unclustered pathways). We can now see
clear themes in the data pertaining to negative *FASN* genetic interactions,
such as `diseases glycosylation, proteins`, `golgi transport`, and
`rab regulation trafficking`. These can be compared and constrasted with the
enrichment seen for *FASN* positive interactions.
Try this out yourself! Hopefully it’s the only fedup you achieve
:grimacing:
# Versioning
For the versions available, see the [tags on this
repo](https://github.com/rosscm/fedup/tags).
# Shoutouts
:sparkles:[**2020**](https://media.giphy.com/media/z9AUvhAEiXOqA/giphy.gif):sparkles:
rosscm/fedup documentation built on July 19, 2021, 2:21 a.m.
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knitr::opts_chunk$set( collapse = TRUE, comment = "#>", fig.path = "inst/figures/", out.width = "100%" )
**F**isher's Test for **E**nrichment and **D**epletion of **U**ser-Defined **P**athways
[](https://ci.appveyor.com/project/rosscm/fedup)
[](https://codecov.io/gh/rosscm/fedup)
[](https://ci.appveyor.com/project/rosscm/fedup)
[](https://codecov.io/gh/rosscm/fedup)
`fedup` is an R package that tests for enrichment and depletion of user-defined
pathways using a Fisher's exact test. The method is designed for versatile
pathway annotation formats (eg. gmt, txt, xlsx) to allow the user to run
pathway analysis on custom annotations. This package is also
integrated with Cytoscape to provide network-based pathway visualization
that enhances the interpretability of the results.
This README will quickly demonstrate how to use `fedup` when testing two
sets of genes. Refer to full
[vignettes](https://www.bioconductor.org/packages/release/bioc/html/fedup.html)
for additional information and implementations (e.g., using single or
multiple test sets).
# Contents
- [System prerequisites](#system-prerequisites)
- [Installation](#installation)
- [Running the package](#running-the-package)
* [Input data](#input-data)
* [Pathway analysis](#pathway-analysis)
* [Dot plot](#dot-plot)
* [Enrichment map](#enrichment-map)
- [Versioning](#versioning)
- [Shoutouts](#shoutouts)
# System prerequisites
**R version** ≥ 4.1
**R packages**:
- **CRAN**: openxlsx, tibble, dplyr, data.table, ggplot2, ggthemes,
forcats, RColorBrewer
- **Bioconductor**: RCy3
# Installation
Install `fedup` from Bioconductor:
wzxhzdk:1
Or install the development version from Github:
wzxhzdk:2
Load necessary packages:
wzxhzdk:3
# Running the package
## Input data
Load test genes (`geneDouble`) and pathway annotations (`pathwaysGMT`):
wzxhzdk:4
Take a look at the data structure:
wzxhzdk:5
To see more info on this data, run `?geneDouble` or `?pathwaysGMT`.
You could also run `example("prepInput", package = "fedup")` or
`example("readPathways", package = "fedup")` to see exactly how the
data was generated using the `prepInput()` and `readPathways()` functions.
`?` and `example()` can be used on any other functions mentioned here to
see their documentation and run examples.
## Pathway analysis
Now use `runFedup` on the sample data:
wzxhzdk:6
The `fedupRes` output is a list of length `length(which(names(geneDouble) !=
"background"))`, corresponding to the number of test sets in `geneDouble`
(i.e., 2).
View `fedup` results for `FASN_negative` sorted by pvalue:
wzxhzdk:7
Let's also view `fedup` results for `FASN_positive`, sorted by pvalue:
wzxhzdk:8
## Dot plot
Prepare data for plotting via `dplyr` and `tidyr`:
wzxhzdk:9
Plot significant results (qvalue < 0.05) in the form of a dot plot via
`plotDotPlot`. Colour and facet the points by the `sign` column:
wzxhzdk:10
Look at all those chick... enrichments! This is a bit overwhelming, isn't it?
How do we interpret these 156 fairly redundant pathways in a way that doesn't
hurt our tired brains even more? Oh I know, let's use an enrichment map!
## Enrichment map
First, make sure to have
[Cytoscape](https://cytoscape.org/download.html) downloaded and and open
on your computer. You’ll also need to install the
[EnrichmentMap](http://apps.cytoscape.org/apps/enrichmentmap) (≥ v3.3.0) and
[AutoAnnotate](http://apps.cytoscape.org/apps/autoannotate) apps.
Then format results for compatibility with EnrichmentMap using `writeFemap`:
wzxhzdk:11
Prepare a pathway annotation file (gmt format) from the pathway list you
passed to `runFedup` using the `writePathways` function (you don't need to run
this function if your pathway annotations are already in gmt format, but it
doesn't hurt to make sure):
wzxhzdk:12
Cytoscape is open right? If so, run these lines and let the `plotFemap`
magic happen:
wzxhzdk:13
To note here, the EM nodes were coloured manually (by the same colours passed to
`plotDotPlot`) in Cytoscape via the *Change Colors* option in the EM panel.
A feature for automated dataset colouring is set to be released in
[version 3.3.2](https://github.com/BaderLab/EnrichmentMapApp/issues/455)
of EnrichmentMap.
This has effectively summarized the 156 pathways from our dot plot into 21
unique biological themes (including 4 unclustered pathways). We can now see
clear themes in the data pertaining to negative *FASN* genetic interactions,
such as `diseases glycosylation, proteins`, `golgi transport`, and
`rab regulation trafficking`. These can be compared and constrasted with the
enrichment seen for *FASN* positive interactions.
Try this out yourself! Hopefully it’s the only fedup you achieve
:grimacing:
# Versioning
For the versions available, see the [tags on this
repo](https://github.com/rosscm/fedup/tags).
# Shoutouts
:sparkles:[**2020**](https://media.giphy.com/media/z9AUvhAEiXOqA/giphy.gif):sparkles:
rosscm/fedup documentation built on July 19, 2021, 2:21 a.m.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
Embedding an R snippet on your website
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.