BATgate: BATgate: An algorithm for automated flow cytometry analysis...

In saritaupatil/AutoBAT: Automated Analysis of Basophil Activation Testing Flow Cytometry Data

Description

BATgate: An algorithm for automated flow cytometry analysis of basophil activation data

Usage

BATgate(workingdir, fluorophores, SSCno, medcontroltube)

Arguments

fluorophores	List of fluorophores, ordered the same as in the fset
SSCno	The number of clusters within the SSC distribution
medcontroltube	The sample number containing medium only or the control condition in the BAT assay, used to calculate the MFI for definition of CD63hi basophils
wkdir	The location of the working directory, where all of the .fcs files for a BAT experiment are located

Value

This function allows for data-driven flow cytometry analysis of basophil activation data

A csv file containing basophil flow cytometry output including percentage of CD63hi basophils, A pdf file containing graphical gating of flow cytometry analysis for visual inspection

Author(s)

Sarita Patil, [e-mail]

Examples

BATgate(wkdir, c("Time","FSC-A","FSC-H","SSC-A","CD63","CCR3"), 2, 1)
BATgate()

saritaupatil/AutoBAT documentation built on May 29, 2019, 1:25 p.m.