ct_plot_obs: Plot observed concentration-time data

View source: R/ct_plot_obs.R

ct_plot_obsR Documentation

Plot observed concentration-time data

Description

ct_plot_obs is meant to be used in conjunction with extractObsConcTime or extractObsConcTime_mult to create single graphs with overlaid observed concentration-time data.

Usage

ct_plot_obs(
  ct_dataframe,
  mean_type = "arithmetic",
  nominal_times = NA,
  linear_or_log = "semi-log",
  normalize_by_dose = FALSE,
  colorBy_column,
  color_labels = NA,
  legend_label_color = NA,
  color_set = "default",
  obs_shape = NA,
  obs_color = NA,
  obs_size = NA,
  obs_fill_trans = NA,
  obs_line_trans = 0.5,
  connect_obs_points = TRUE,
  indiv_on_top = FALSE,
  include_errorbars = FALSE,
  errorbar_width = 0.5,
  linetype_column,
  linetype_labels = NA,
  linetypes = c("solid", "dashed"),
  line_width = NA,
  line_transparency = NA,
  legend_label_linetype = NA,
  facet1_column,
  facet1_title = NA,
  facet2_column,
  facet2_title = NA,
  facet_ncol = NA,
  facet_nrow = NA,
  floating_facet_scale = FALSE,
  facet_spacing = NA,
  time_range = NA,
  x_axis_interval = NA,
  x_axis_label = NA,
  pad_x_axis = TRUE,
  pad_y_axis = TRUE,
  y_axis_limits_lin = NA,
  y_axis_limits_log = NA,
  y_axis_interval = NA,
  y_axis_label = NA,
  hline_position = NA,
  hline_style = "red dotted",
  vline_position = NA,
  vline_style = "red dotted",
  name_clinical_study = NA,
  graph_labels = TRUE,
  graph_title = NA,
  graph_title_size = 14,
  legend_position = NA,
  legend_orientation = NA,
  prettify_compound_names = TRUE,
  save_graph = NA,
  fig_height = NA,
  fig_width = NA
)

Arguments

ct_dataframe

the input concentration-time data generated by running the function extractConcObsTime_mult or extractObsConcTime. Not quoted.

mean_type

plot "arithmetic" (default) or "geometric" mean concentrations or "median" concentrations as the main (thickest or only) line for each data set. If you don't want to show the means, set this to "none". If you only want to show the mean data and not the individual, set this to whatever mean type you want plus "only", e.g., "arithmetic only" to show only the arithmetic mean data and none of the individuals. If you haven't included mean data in ct_dataframe, the mean will be calculated for you using our best guess as to what the groups should be. In other words, you may want to calculate the means yourself just to be sure you're getting what you expect. Talk to Laura Sh. if you would like help here.

nominal_times

optionally specify what the nominal times were for the concentration-time data. If left as NA, when we calculate the means, we'll group the data by the times exactly as listed in the individual data. If you know that the nominal times were, say, 0, 0.5, 1, 2, and 4 hours but that the times listed in the data were the exact draw times, then supply a numeric vector like this: nominal_times = c(0, 0.5, 1, 2, 4) and we will match each individual time with the nominal time closest to it. This way, you'll get data from a draw time at, say, 4.1 h grouped with another draw time at, say, 3.95 h; they'll both be put into the 4-hour bin.

linear_or_log

the type of graph to be returned. Options:

"semi-log"

y axis is log transformed; this is the default

"linear"

no axis transformation

"both vertical"

both the linear and the semi-log graphs will be returned, and graphs are stacked vertically

"both horizontal"

both the linear and the semi-log graphs will be returned, and graphs are stacked horizontally

normalize_by_dose

TRUE or FALSE (default) for whether to show dose-normalized concentration-time profiles

colorBy_column

(optional) the column in ct_dataframe that should be used for determining which color lines and/or points will be. This should be unquoted, e.g., colorBy_column = Tissue.

color_labels

optionally specify a character vector for how you'd like the labels for whatever you choose for colorBy_column to show up in the legend. For example, use color_labels = c("file 1.xlsx" = "fa 0.5", "file 2.xlsx" = "fa 0.2") to indicate that "file 1.xlsx" is for an fa of 0.5 and "file 2.xlsx" is for an fa of 0.2. The order in the legend will match the order designated here.

legend_label_color

optionally indicate on the legend something explanatory about what the colors represent. For example, if colorBy_column = File and legend_label_color = "Simulations with various fa values", that will make the label above the file names in the legend more explanatory than just "File". The default is to use whatever the column name is for colorBy_column. If you don't want a label for this legend item, set this to "none".

color_set

the set of colors to use. Options:

"default"

a set of colors from Cynthia Brewer et al. from Penn State that are friendly to those with red-green colorblindness. The first three colors are green, orange, and purple. This can also be referred to as "Brewer set 2". If there are only two unique values in the colorBy_column, then Brewer set 1 will be used since red and blue are still easily distinguishable but also more aesthetically pleasing than green and orange.

"Brewer set 1"

colors selected from the Brewer palette "set 1". The first three colors are red, blue, and green.

"ggplot2 default"

the default set of colors used in ggplot2 graphs (ggplot2 is an R package for graphing.)

"rainbow"

colors selected from a rainbow palette. The default palette is limited to something like 6 colors, so if you have more than that, that's when this palette is most useful. It's not very useful when you only need a couple of colors.

"blue-green"

a set of blues fading into greens. This palette can be especially useful if you are comparing a systematic change in some continuous variable – for example, increasing dose or predicting how a change in intrinsic solubility will affect concentration-time profiles – because the direction of the trend will be clear.

"blues"

a set of blues fading light blue to dark blue. Like "blue-green", this palette can be especially useful if you are comparing a systematic change in some continuous variable.

"Tableau"

uses the standard Tableau palette; requires the "ggthemes" package

"viridis"

from the eponymous package by Simon Garnier and ranges colors from purple to blue to green to yellow in a manner that is "printer-friendly, perceptually uniform and easy to read by those with colorblindness", according to the package author

a character vector of colors

If you'd prefer to set all the colors yourself to exactly the colors you want, you can specify those colors here. An example of how the syntax should look: color_set = c("dodgerblue3", "purple", "#D8212D") or, if you want to specify exactly which item in colorBy_column gets which color, you can supply a named vector. For example, if you're coloring the lines by the compound ID, you could do this: color_set = c("substrate" = "dodgerblue3", "inhibitor 1" = "purple", "primary metabolite 1" = "#D8212D"). If you'd like help creating a specific gradation of colors, please talk to a member of the R Working Group about how to do that using colorRampPalette.

obs_shape

optionally specify what shapes are used to depict observed data for a) the substrate drug alone and b) the substrate drug in the presence of a perpetrator. Input should look like this, for example: c(1, 2) to get an open circle for the substrate and an open triangle for the substrate in the presence of perpetrators, if there are any. If you only specify one value, it will be used for both substrate with and without perpetrators. To see all the possible shapes and what number corresponds to which shape, type ggpubr::show_point_shapes() into the console. If left as NA, substrate alone will be an open circle and substrate + inhibitor 1 will be an open triangle.

obs_color

optionally specify a color to use for observed data if the color isn't already mapped to a specific column. By default, observed data will be the same color as whatever else matches those observed data in colorBy_column, so if you have colored by compound ID, for example, the observed data will also be colored by compound ID. If you have one observed file that you're comparing to multiple simulation files (this is what ct_plot_overlay will do if "File" is NA for the observed data), then the observed data will all be black by default, or you could set that color to be, say, a lovely purple by setting this: obs_color = "darkorchid4". Hex color codes are also ok to use, and setting this to "none" will remove observed data from the graph.

obs_size

optionally specify the size of the points to use for the observed data. If left as NA, the size will be 2.

obs_fill_trans

optionally specify the transparency for the fill of the observed data points, which can be helpful when you have a lot of points overlapping. This only applies when you have specified a value for obs_color and when obs_shape is a shape that has a fill (example: obs_shape = 21 for a filled circle, which is the default). Acceptable values are from 0 (fully transparent, so no fill at all) to 1 (completely opaque or black). If left as the default NA, the observed data points will be 50 percent transparent, so the same as if this were set to 0.5.

obs_line_trans

specify the transparency for the outline of the observed data points, which can be helpful when you have a lot of points overlapping. Acceptable values are from 0 (fully transparent, so no line at all) to 1 (completely opaque or black), and the default is 0.5 for 50 transparent.

connect_obs_points

TRUE (default) or FALSE for whether to add connecting lines between observed data points from the same individual

indiv_on_top

TRUE (default) or FALSE for whether to show the individual observed data on top of the mean observed data. If FALSE, the mean data will be on top.

include_errorbars

TRUE or FALSE (default) for whether to include error bars for observed data points. This ONLY applies when you have supplied observed data from V22 or higher because those data files included a column titled "SD/SE", which is what we'll use for determining the error bar heights.

errorbar_width

width of error bars to use in hours (or, if you've used some other time unit, in whatever units are in your data). Default is 0.5.

linetype_column

the column in ct_dataframe that should be used for determining the line types and also the shapes of the points for depicting any observed data. For example, if linetype_column is set to Inhibitor, then the default is to show a solid line (simulated data) and an open circle (observed data) for no inhibitor being present and then a dashed line (simulated data) and an open triangle (observed data) when the inhibitor is present. You can set which types of lines to use with the argument linetypes and you can set which shapes of points you want with the argument obs_shape.

linetype_labels

optionally specify a character vector for how you'd like the labels for whatever you choose for linetype_column to show up in the legend. For example, use linetype_labels = c("file 1.xlsx" = "fa 0.5", "file 2.xlsx" = "fa 0.2") to indicate that "file 1.xlsx" is for an fa of 0.5 and "file 2.xlsx" is for an fa of 0.2. The order in the legend will match the order designated here.

linetypes

the line types to use. Default is "solid" for all lines. You'll need one line type for each possible value in the column you specified for linetype_column. If you get a graph you didn't expect as far as line types go, try checking what all the possible values are for the column you specified for linetype_column. You can do this by checking, e.g., unique(CT$Inhibitor) if your ct_dataframe was named "CT" and the column you set for linetype_column was "Inhibitor". To see possible line types by name, please enter ggpubr::show_line_types() into the console.

line_width

optionally specify how thick to make the lines. Acceptable input is a number; the default is 1 for most lines and 0.8 for some, to give you an idea of where to start.

line_transparency

optionally specify the transparency for the trial mean or percentile lines. Acceptable values are from 0 (fully transparent, so no line at all) to 1 (completely opaque or black). If left as the default NA, this value will be automatically determined.

legend_label_linetype

optionally indicate on the legend something explanatory about what the line types represent. For example, if linetype_column = Inhibitor and legend_label_linetype = "Inhibitor present", that will make the label in the legend above, e.g., "none", and whatever perpetrator was present more explanatory than just "Inhibitor". The default is to use whatever the column name is for linetype_column. If you don't want a label for this legend item, set this to "none".

facet1_column

optionally break up the graph into small multiples; this specifies the first of up to two columns to break up the data by, and the designated column name should be unquoted, e.g., facet1_column = Tissue. If floating_facet_scale is FALSE and you haven't specified facet_ncol or facet_nrow, then facet1_column will designate the rows of the output graphs.

facet1_title

optionally specify a title to describe facet 1. This is ignored if floating_facet_scale is TRUE or if you have specified facet_ncol or facet_nrow.

facet2_column

optionally break up the graph into small multiples; this specifies the second of up to two columns to break up the data by, and the designated column name should be unquoted, e.g., facet2_column = CompoundID. If floating_facet_scale is FALSE and you haven't specified facet_ncol or facet_nrow, then facet2_column will designate the columns of the output graphs.

facet2_title

optionally specify a title to describe facet 2. This is ignored if floating_facet_scale is TRUE or if you have specified facet_ncol or facet_nrow.

facet_ncol

optionally specify the number of columns of facetted graphs you would like to have. This only applies when you have specified a column for facet1_column and/or facet2_column.

facet_nrow

optionally specify the number of rows of facetted graphs you would like to have. This only applies when you have specified a column for facet1_column and/or facet2_column.

floating_facet_scale

TRUE or FALSE (default) for whether to allow the axes for each facet of a multi-facetted graph to scale freely to best fit whatever data are present. Default is FALSE, which means that all data will be on the same scale for easy comparison. However, this could mean that some graphs have lines that are hard to see, so you can set this to TRUE to allow the axes to shrink or expand according to what data are present for that facet. Floating axes comes with a trade-off for the looks of the graphs, though: Setting this to TRUE does mean that your x axis won't automatically have pretty breaks that are sensible for times in hours.

facet_spacing

Optionally set the spacing between facets. If left as NA, a best-guess as to a reasonable amount of space will be used. Units are "lines", so try, e.g. facet_spacing = 2. (Reminder: Numeric data should not be in quotes.)

time_range

time range to display. Options:

NA

entire time range of data; default

a start time and end time in hours

only data in that time range, e.g. c(24, 48). Note that there are no quotes around numeric data.

"first dose"

only the time range of the first dose

"last dose"

only the time range of the last dose

"penultimate dose"

only the time range of the 2nd-to-last dose, which can be useful for BID data where the end of the simulation extended past the dosing interval or data when the substrate was dosed BID and the perpetrator was dosed QD

a specific dose number with "dose" or "doses" as the prefix

the time range encompassing the requested doses, e.g., time_range = "dose 3" for the 3rd dose or time_range = "doses 1 to 4" for doses 1 to 4

"all obs" or "all observed" if you feel like spelling it out

Time range will be limited to only times when observed data are present.

"last dose to last observed" or "last obs" for short

Time range will be limited to the start of the last dose until the last observed data point.

x_axis_interval

set the x-axis major tick-mark interval. Acceptable input: any number or leave as NA to accept default values, which are generally reasonable guesses as to aesthetically pleasing and PK-relevant intervals.

x_axis_label

optionally supply a character vector or an expression to use for the x axis label

pad_x_axis

optionally add a smidge of padding to the x axis (default is TRUE, which includes some generally reasonable padding). If changed to FALSE, the y axis will be placed right at the beginning of your time range and all data will end exactly at the end of the time range specified. If you want a specific amount of x-axis padding, set this to a number; the default is c(0.02, 0.04), which adds 2% more space to the left side and 4% more space to the right side of the x axis. If you only specify one number, padding is added to the left side.

pad_y_axis

optionally add a smidge of padding to the y axis (default is TRUE, which includes some generally reasonable padding). As with pad_x_axis, if changed to FALSE, the x axis will be placed right at the bottom of your data, possibly cutting a point in half. If you want a specific amount of y-axis padding, set this to a number; the default is c(0.02, 0), which adds 2% more space to the bottom and nothing to the top of the y axis. If you only specify one number, padding is added to the bottom.

y_axis_limits_lin

Optionally set the Y axis limits for the linear plot, e.g., c(10, 1000). If left as NA, the Y axis limits for the linear plot will be automatically selected. This only applies when you have requested a linear plot with linear_or_log.

y_axis_limits_log

Optionally set the Y axis limits for the semi-log plot, e.g., c(10, 1000). Values will be rounded down and up, respectively, to the nearest order of magnitude. If left as NA, the Y axis limits for the semi-log plot will be automatically selected. This only applies when you have requested a semi-log plot with linear_or_log.

y_axis_interval

set the y-axis major tick-mark interval. Acceptable input: any number or leave as NA to accept default values, which are generally reasonable guesses as to aesthetically pleasing intervals.

y_axis_label

optionally supply a character vector or an expression to use for the y axis label

hline_position

numerical position(s) of any horizontal lines to add to the graph. The default is NA to have no lines, and good syntax if you do want lines would be, for example, hline_position = 10 to have a horizontal line at 10 ng/mL (or whatever your concentration units are) or hline_position = c(10, 100, 1000) to have horizontal lines at each of those y values. Examples of where this might be useful would be to indicate a toxicity threshold, a target Cmin, or the lower limit of quantification for the assay used to generate the concentration-time data.

hline_style

the line color and type to use for any horizontal lines that you add to the graph with hline_position. Default is "red dotted", but any combination of 1) a color in R and 2) a named linetype is acceptable. Examples: "red dotted", "blue dashed", or "#FFBE33 longdash". To see all the possible linetypes, type ggpubr::show_line_types() into the console.

vline_position

numerical position(s) of any vertical lines to add to the graph. The default is NA to have no lines, and good syntax if you do want lines would be, for example, vline_position = 12 to have a vertical line at 12 h or vline_position = seq(from = 0, to = 168, by = 24) to have horizontal lines every 24 hours for one week. Examples of where this might be useful would be indicating dosing times or the time at which some other drug was started or stopped.

vline_style

the line color and type to use for any vertical lines that you add to the graph with vline_position. Default is "red dotted", but any combination of 1) a color in R and 2) a named linetype is acceptable. Examples: "red dotted", "blue dashed", or "#FFBE33 longdash". To see all the possible linetypes, type ggpubr::show_line_types() into the console.

name_clinical_study

optionally specify the name(s) of the clinical study or studies for any observed data. This only affects the caption of the graph. For example, specifying name_clinical_study = "101, fed cohort" will result in a figure caption that reads in part "clinical study 101, fed cohort". If you have more than one study, that's fine; we'll take care of stringing them together appropriately. Just list them as a character vector, e.g., name_clinical_study = c("101", "102", "103") will become "clinical studies 101, 102, and 103."

graph_labels

TRUE or FALSE for whether to include labels (A, B, C, etc.) for each of the small graphs. (Not applicable if only outputting linear or only semi-log graphs.)

graph_title

optionally specify a title that will be centered across your graph or set of graphs

graph_title_size

the font size for the graph title if it's included; default is 14. This also determines the font size of the graph labels.

legend_position

Specify where you want the legend to be. Options are "left", "right" (default in most scenarios), "bottom", "top", or "none" if you don't want one at all.

legend_orientation

optionally specify how the legend entries should be oriented. Options are "vertical" or "horizontal", and, if left as NA, the legend entries will be "vertical" when the legend is on the left or right and "horizontal" when it's on the top or bottom.

prettify_compound_names

set this to a) TRUE (default) or FALSE for whether to make the compound names in the legend prettier or b) supply a named character vector to set it to the exact name you'd prefer to see in your legend. For example, prettify_compound_names = c("Sim-Ketoconazole-400 mg QD" = "ketoconazole", "Wks-Drug ABC-low_ka" = "Drug ABC") will make those compounds "ketoconazole" and "Drug ABC" in a legend, and prettify_compound_names = TRUE will make some reasonable guesses about what a prettier compound name should be. An example of setting this to TRUE: "SV-Rifampicin-MD" would become "rifampicin", and "Sim-Ketoconazole-200 mg BID" would become "ketoconazole".

save_graph

optionally save the output graph by supplying a file name in quotes here, e.g., "My conc time graph.png"or "My conc time graph.docx". The nice thing about saving to Word is that the figure title and caption text will be partly filled in automatically, although you should check that the text makes sense in light of your exact graph. If you leave off ".png" or ".docx", it will be saved as a png file, but if you specify a different graphical file extension, it will be saved as that file format. Acceptable graphical file extensions are "eps", "ps", "jpeg", "jpg", "tiff", "png", "bmp", or "svg". Do not include any slashes, dollar signs, or periods in the file name. Leaving this as NA means the file will not be automatically saved to disk.

fig_height

figure height in inches

fig_width

figure width in inches

Value

returns a ggplot2 graph

Examples

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shirewoman2/Consultancy documentation built on Feb. 18, 2025, 10 p.m.