coa: CoA data from PureHoney.

In stevehoang/assayr: Tools for analyzing biochemical assay data.

Description

Analyzed data from PureHoney plates 541 and 542, this data has been through the QC process and is ready to be analyzed and plotted for results visualization.

Usage

coa

Format

A data frame with 30 variables:

run

study run id

sample_id

study level unique sample id

cell_type

type of cell

donor

cell donor name

donor_pheno

phenotype of cell donor

media

base media used

tx_run

paste of tx_cmpd and run

tx_treat

challenge condition

tx_cmpd

compound id

tx_conc

concentration of tx_cmp in uM

imaging_plate

culture/imaging plate id

imaging_well

culture/imaging well id

assay_plate

assay plate id

assay_prun

assay read/run id

assay_well

assay well id

plate_run

paste of assay_plate and assay_prun

contents

assay_plate level sample id

targ

analyte/target measured

iso_label

is targ isotopicly labeled?

curve_id

standard curve used

curve_plot

targ base name, used for plotting both species together

conc

concentration targ in nM

in_range

is conc in the range of detection?

recov

fraction of internal standard measured

conc_corrected

conc / recov

nuc_well

count of nuclei per imaging_plate:imaging_well

cell_vol_well_L

nuc_well * (3.4 * 10^-12) for hepatocytes

conc_nmols

conc * (.0001 + cell_volume_well_L)

conc_incell_nM

conc_nmols / cell_volume_well_L

conc_incell_uM

conc_incell_nM / 1000

For futher details talk to Nate ([e-mail])

stevehoang/assayr documentation built on May 24, 2019, 7:20 a.m.