read_incu: read incucyte metrics

In uhlitz/incucyter: Analysing IncuCyte ZOOM® data in R

Description

read_incu() reads incucyte metrics. Please provide an annotation table for downstream analyses.

Usage

read_incu(file, annotation = NULL, delay = 0, per_image = T)

Arguments

file	Metrics file exported with IncuCyte ZOOM<c2><ae> software.
annotation	Annotation file with at least four columns: Analysis_Job (e.g. "Experiment_I"), Well (e.g. "A1"), Treatment (e.g. "ctrl") and Reference (can be multiple e.g. "A1,B1"), and one row per well.
delay	Time between treatment and first image acquisition in minutes (default 0).
per_image	IncuCyte ZOOM<c2><ae> can export values per image or export mean values per well. Hence, set per_image = T or F depending on your export settings.

Value

A tibble of incucyte metrics.

Examples

read_incu(file = system.file("extdata", "sample_data_GO_Confluence_percent.txt", package = "incucyter"),
annotation = system.file("extdata", "sample_data_annotation.tsv", package = "incucyter"))

uhlitz/incucyter documentation built on May 3, 2019, 2:23 p.m.