inst/helpers/regressFeatures.md

In whtns/seuratTools: Tools for Managing Seurat Objects

Regress Features

Often we would like to correct for expression variation in our analysis due to phenomena unrelated to our focus of study. Such unwanted effects can include cell-cycle variability or differences in mitochondrial expression.

Rather than simply excluding count values attributable to a given process, it is better to adjust the expression of all remaining genes or transcripts in each cell based on the sum score of relevant genes, to regress out cell-cycle effects, for example

Such regression can be performed for any arbitrary gene set. Some preset gene lists are included in seuratTools including cell-cycle, mitochondrial, and apoptotsis related gene sets.

whtns/seuratTools documentation built on June 28, 2024, 8:30 p.m.