Description Usage Arguments Value
Read autoradiographic intensities from multiple lanes in a single gel. NB: add specifics about the technology.
1 | read_IPdata(dir_raw, dir_raw_marked = NULL)
|
dir_raw |
Path to IP data file ( |
dir_raw_marked |
Default is |
dat_raw
A data frame for raw autoragraphical intensities, one row per
location along the original gel scan. The first column records the location along
the gel that linearly increase from 0 to 1; the second column and beyond are
radio-intensity readings obtained from the tested samples. The Lane 1 (in the second column)
intensities are observed from the reference sample comprising 7 known
molecules of distinct weights (200, 116, 97, 66, 45, 31, 21.5 kDa).
dat_raw_marked
A data frame with one hand-picked band per row.
At least three columns are expected:
Lane Index (from 1 to the total no. of tested samples),
Location along the gel (a value between 0 and 1), and
Intensity value (positive numeric value)
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