Data, extracted from scanned images using Agilent Feature Extraction Software, are stored in a uRNAList object.
A data example is provided. The data example includes 3 experimental conditions with two replicates.
For these data, chips were scanned using the Agilent G2567AA Microarray Scanner System (Agilent Technologies) Image analysis and data collection were carried out using the Agilent Feature Extraction 220.127.116.11. (AFE).
Data, colected with the Agilent Feature Extraction Software, are stored in a uRNAList object with the following components:
vector of characters, 'AGilent Probe Name'
vector of characters, 'microRNA Name'
vector of integers, '0'= Feature, '1'= Positive control, '-1'= Negative control
matrix, FLAG to classify signal if 'IsGeneDetected=1' or 'not=0'
matrix, FLAG to classify signal if 'IsSaturated = 1' or 'not=0'
matrix, FLAG to classify signal if 'IsFeatPopnOL = 0' or 'not=1'
matrix, FLAG to classify signal if 'gIsFeatNonUnifOL = 0' or 'not=1'