The FRASER packages contains an example dataset that is a subset of the Kremer et al study. The main purpose of those files are to demonstrate the functionality of the FRASER package.
The three BAM files in
inst/extdata/bam belong to one of the diagnost
individuals from the study. In short, they contain Illumina RNA-seq data
from fibroblast samples and aligned with STAR against the UCSC hg19 assembly.
For this package only reads spanning the genes
extracted. More details on the protocol can be found in the
The split-read and splice-site-read count matrices
inst/extdata/raw_site_counts.tsv.gz) are also a subset of the
Kremer et al study. Here, the
individuals with diagnosed splice defects and some controls are picked.
The counts are extracted from the BAM files with the function
FRASER::countRNAData. Again it contains only a subset of count for
Both datasets are accompanied by a pseudo anonymised sample annotation.
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