dataProcessPlots: Visualization for explanatory data analysis

In MSstats: Protein Significance Analysis in DDA, SRM and DIA for Label-free or Label-based Proteomics Experiments

Description

To illustrate the quantitative data after data-preprocessing and quality control of MS runs, dataProcessPlots takes the quantitative data from function (dataProcess) as input and automatically generate three types of figures in pdf files as output : (1) profile plot (specify "ProfilePlot" in option type), to identify the potential sources of variation for each protein; (2) quality control plot (specify "QCPlot" in option type), to evaluate the systematic bias between MS runs; (3) mean plot for conditions (specify "ConditionPlot" in option type), to illustrate mean and variability of each condition per protein.

Usage

dataProcessPlots(data=data,
		type=type,
		featureName="Transition",
		ylimUp=FALSE,
		ylimDown=FALSE,
		scale=FALSE,
		interval="CI",
		x.axis.size=10,
		y.axis.size=10,
		text.size=4,
		text.angle=0,
		legend.size=7,
		dot.size.profile=2,
		dot.size.condition=3,
		width=10, 
		height=10, 
		which.Protein="all",
		originalPlot=TRUE, 
		summaryPlot=TRUE, 
		save_condition_plot_result=FALSE,
		remove_uninformative_feature_outlier=FALSE,
		address="")

Arguments

data	name of the (output of dataProcess function) data set.
type	choice of visualization. "ProfilePlot" represents profile plot of log intensities across MS runs. "QCPlot" represents quality control plot of log intensities across MS runs. "ConditionPlot" represents mean plot of log ratios (Light/Heavy) across conditions.
featureName	for "ProfilePlot" only, "Transition" (default) means printing feature legend in transition-level; "Peptide" means printing feature legend in peptide-level; "NA" means no feature legend printing.
ylimUp	upper limit for y-axis in the log scale. FALSE(Default) for Profile Plot and QC Plot use the upper limit as rounded off maximum of log2(intensities) after normalization + 3. FALSE(Default) for Condition Plot is maximum of log ratio + SD or CI.
ylimDown	lower limit for y-axis in the log scale. FALSE(Default) for Profile Plot and QC Plot is 0. FALSE(Default) for Condition Plot is minumum of log ratio - SD or CI.
scale	for "ConditionPlot" only, FALSE(default) means each conditional level is not scaled at x-axis according to its actual value (equal space at x-axis). TRUE means each conditional level is scaled at x-axis according to its actual value (unequal space at x-axis).
interval	for "ConditionPlot" only, "CI"(default) uses confidence interval with 0.95 significant level for the width of error bar. "SD" uses standard deviation for the width of error bar.
x.axis.size	size of x-axis labeling for "Run" in Profile Plot and QC Plot, and "Condition" in Condition Plot. Default is 10.
y.axis.size	size of y-axis labels. Default is 10.
text.size	size of labels represented each condition at the top of graph in Profile Plot and QC plot. Default is 4.
text.angle	angle of labels represented each condition at the top of graph in Profile Plot and QC plot or x-axis labeling in Condition plot. Default is 0.
legend.size	size of feature legend (transition-level or peptide-level) above graph in Profile Plot. Default is 7.
dot.size.profile	size of dots in profile plot. Default is 2.
dot.size.condition	size of dots in condition plot. Default is 3.
width	width of the saved file. Default is 10.
height	height of the saved file. Default is 10.
which.Protein	Protein list to draw plots. List can be names of Proteins or order numbers of Proteins from levels(data$ProcessedData$PROTEIN). Default is "all", which generates all plots for each protein. For QC plot, "allonly" will generate one QC plot with all proteins.
originalPlot	TRUE(default) draws original profile plots.
summaryPlot	TRUE(default) draws profile plots with summarization for run levels.
save_condition_plot_result	TRUE saves the table with values using condition plots. Default is FALSE.
remove_uninformative_feature_outlier	It only works after users used featureSubset="highQuality" in dataProcess. TRUE allows to remove 1) the features are flagged in the column, feature_quality="Uninformative" which are features with bad quality, 2) outliers that are flagged in the column, is_outlier=TRUE in Profile plots. FALSE (default) shows all features and intensities in profile plots.
address	the name of folder that will store the results. Default folder is the current working directory. The other assigned folder has to be existed under the current working directory. An output pdf file is automatically created with the default name of "ProfilePlot.pdf" or "QCplot.pdf" or "ConditionPlot.pdf" or "ConditionPlot_value.csv". The command address can help to specify where to store the file as well as how to modify the beginning of the file name. If address=FALSE, plot will be not saved as pdf file but showed in window.

Details

• Profile Plot : identify the potential sources of variation of each protein. QuantData$ProcessedData is used for plots. X-axis is run. Y-axis is log-intensities of transitions. Reference/endogenous signals are in the left/right panel. Line colors indicate peptides and line types indicate transitions. In summarization plots, gray dots and lines are the same as original profile plots with QuantData$ProcessedData. Dark dots and lines are for summarized intensities from QuantData$RunlevelData.

• QC Plot : illustrate the systematic bias between MS runs. After normalization, the reference signals for all proteins should be stable across MS runs. QuantData$ProcessedData is used for plots. X-axis is run. Y-axis is log-intensities of transition. Reference/endogenous signals are in the left/right panel. The pdf file contains (1) QC plot for all proteins and (2) QC plots for each protein separately.

• Condition Plot : illustrate the systematic difference between conditions. Summarized intensnties from QuantData$RunlevelData are used for plots. X-axis is condition. Y-axis is summarized log transformed intensity. If scale is TRUE, the levels of conditions is scaled according to its actual values at x-axis. Red points indicate the mean for each condition. If interval is "CI", blue error bars indicate the confidence interval with 0.95 significant level for each condition. If interval is "SD", blue error bars indicate the standard deviation for each condition.The interval is not related with model-based analysis.

The input of this function is the quantitative data from function (dataProcess).

Value

pdf will be generated under the working directory.

Author(s)

Meena Choi, Olga Vitek.

Maintainer: Meena Choi ([e-mail])

References

Meena Choi, Ching-Yun Chang, Timothy Clough, Daniel Broudy, Trevor Killeen, Brendan MacLean and Olga Vitek. "MSstats: an R package for statistical analysis of quantitative mass spectrometry-based proteomic experiments" Bioinformatics, 30(17):2524-2526, 2014.

Ching-Yun Chang, Paola Picotti, Ruth Huttenhain, Viola Heinzelmann-Schwarz, Marko Jovanovic, Ruedi Aebersold, Olga Vitek. "Protein significance analysis in selected reaction monitoring (SRM) measurements." Molecular & Cellular Proteomics, 11:M111.014662, 2012.

Timothy Clough, Safia Thaminy, Susanne Ragg, Ruedi Aebersold, Olga Vitek. "Statistical protein quantification and significance analysis in label-free LC-M experiments with complex designs" BMC Bioinformatics, 13:S16, 2012.

Examples

# Consider quantitative data (i.e. QuantData) from a yeast study with ten time points of interests, 
# three biological replicates, and no technical replicates which is a time-course experiment. 
# The goal is to provide pre-analysis visualization by automatically generate two types of figures 
# in two separate pdf files. 
# Protein IDHC (gene name IDP2) is differentially expressed in time point 1 and time point 7, 
# whereas, Protein PMG2 (gene name GPM2) is not.

QuantData<-dataProcess(SRMRawData)
head(QuantData$ProcessedData)

# Profile plot
dataProcessPlots(data=QuantData,type="ProfilePlot")

# Quality control plot 
dataProcessPlots(data=QuantData,type="QCPlot")	

# Quantification plot for conditions
dataProcessPlots(data=QuantData,type="ConditionPlot")

MSstats documentation built on Feb. 28, 2021, 2:01 a.m.