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## ---- eval=FALSE--------------------------------------------------------------
# library(shiny)
# shinyUI(pageWithSidebar(
# # Application title
# headerPanel("Simple Genome Browser"),
# # Sidebar with a slider inputs and selection boxes
# sidebarPanel(
# sliderInput("windowsize",
# "Windowsize:",
# min = 10,
# max = 200,
# value = 50,
# step = 5),
# uiOutput("chromSelect"),
# uiOutput("genomicPositionSelect")
# ),
# # Show a plot of the region
# mainPanel(
# plotOutput("mismatchPlot", height=800)
# )
# ))
## ---- eval=FALSE--------------------------------------------------------------
# library(shiny)
# library(h5vc)
# library(rhdf5)
# tallyFile <- "yeast.hfs5"
# study <- "/yeast"
# h5ls( tallyFile )
# chromosomes <- h5ls( tallyFile )
# chromlengths <- as.numeric(subset( chromosomes, otype == "H5I_DATASET" & name == "Reference" )$dim)
# chromosomes <- subset( chromosomes, otype == "H5I_GROUP" & name != "yeast" )$name
# names(chromlengths) = chromosomes
#
# # Define server logic required to generate and plot a random distribution
# shinyServer(function(input, output) {
#
# output$chromSelect <- renderUI({
# selectInput( "chrom", "Chromosome", choices = c("I","II","III","IV","V","VI","VII","VIII","IX","X","XI","XII","XIII","XIV","XV","XVI", "Mito"))
# })
#
# output$genomicPositionSelect <- renderUI({
# sliderInput( "gpos", "Genomic Position:", min = 10, max = chromlengths[input$chrom] - 10, value = 200 )
# })
#
# group <- reactive({ paste( study, input$chrom, sep="/" ) })
#
# sampleData <- reactive({ sd = getSampleData( tallyFile, group() ); sd$Sample = c("YB210","s288c"); sd })
#
# pos <- reactive({
# min( max( input$windowsize + 1, input$gpos ), chromlengths[input$chrom] - input$windowsize - 1 )
# })
#
# data <- reactive({
# h5dapply(
# tallyFile,
# group(),
# blocksize = input$windowsize*3,
# names = c("Coverages","Counts","Deletions"),
# range = c( max( pos() - input$windowsize, 0 ), min( pos() + input$windowsize, chromlengths[input$chrom] ) )
# )[[1]]
#
# })
#
# output$mismatchPlot <- renderPlot({
# p = mismatchPlot(
# data(),
# sampleData(),
# samples = c("s288c","YB210"),
# input$windowsize,
# pos()
# )
# print(p)
# })
# })
## ---- eval=FALSE--------------------------------------------------------------
# library(rhdf5)
# library(h5vc)
# setwd("~/ShinyApps/Yeast")
# tallyFile <- "yeast.hfs5"
# study <- "/yeast"
# h5ls( tallyFile )
# chromosomes <- h5ls( tallyFile )
# chromosomes <- subset( chromosomes, otype == "H5I_GROUP" & name != "yeast" )$name
# variantCalls <- list()
# for( chrom in chromosomes ){
# group <- paste( study, chrom, sep = "/" )
# sdat <- getSampleData( tallyFile, group )
# sdat$Group <- "yeast"
# variantCalls[[chrom]] <- h5dapply(
# filename = tallyFile,
# group = group,
# blocksize = 100000,
# FUN = callVariantsPairedFancy,
# sampledata = sdat,
# cl = vcConfParams( returnDataPoints = TRUE, minStrandAltSupport = 4 ),
# names = c( "Counts", "Coverages", "Reference" ),
# verbose = TRUE
# )
# }
# for( chrom in chromosomes ){
# variantCalls[[chrom]] <- do.call( rbind, variantCalls[[chrom]] )
# }
# variantCalls <- do.call( rbind, variantCalls )
# rownames(variantCalls) = NULL
# variantCalls$Support = variantCalls$caseCountFwd + variantCalls$caseCountRev
# variantCalls$Coverage = variantCalls$caseCoverageFwd + variantCalls$caseCoverageRev
# variantCalls$AF = variantCalls$Support / variantCalls$Coverage
# save( variantCalls, file = "yeast.variants.RDa" )
## ---- eval=FALSE--------------------------------------------------------------
# library(shiny)
# shinyUI(pageWithSidebar(
#
# # Application title
# headerPanel("Simple Variant Browser - Yeast Strains Example"),
#
# # Sidebar with a slider inputs and selection boxes
# sidebarPanel(
# sliderInput("windowsize",
# "Windowsize:",
# min = 10,
# max = 200,
# value = 50,
# step = 5),
# uiOutput("chromSelect"),
# sliderInput("af",
# "Allele Frequency:",
# min = 0,
# max = 1,
# value = c(0.1,1.0),
# step = 0.01),
# sliderInput( "minSupport",
# "Minimum Support",
# min = 2,
# max = 200,
# value = 2),
# sliderInput( "minCoverage",
# "Minimum Coverage",
# min = 10,
# max = 500,
# value = 50,
# step = 5),
# uiOutput("variantSelect"),
# textOutput("diag")
# ),
#
# # Show a plot of the region
# mainPanel(
# tabsetPanel(
# tabPanel( title = "Region Mismatch Plot", plotOutput("mismatchPlot", height=800) ),
# tabPanel( title = "Variant Table", tableOutput("variantTable") ),
# tabPanel( title = "Variant Summary Plots", plotOutput("afHist") )
# )
# )
# ))
## ---- eval=FALSE--------------------------------------------------------------
# library(shiny)
# library(h5vc)
# library(rhdf5)
# library(ggplot2)
# library(grid)
#
# tallyFile <- "yeast.hfs5"
# study <- "/yeast"
# h5ls( tallyFile )
# chromosomes = h5ls( tallyFile )
# chromlengths = as.numeric(subset( chromosomes, otype == "H5I_DATASET" & name == "Reference" )$dim)
# chromosomes = subset( chromosomes, otype == "H5I_GROUP" & name != "yeast" )$name
# names(chromlengths) = chromosomes
#
# load(file="yeast.variants.RDa")
# variantCalls$start <- variantCalls$start + 1 #fixing difference in counting 0-based vs. 1-based
# variantCalls$end <- variantCalls$end + 1
# # Define server logic required to generate and plot a random distribution
# shinyServer(function(input, output) {
#
# output$chromSelect <- renderUI({
# selectInput( "chrom", "Chromosome", choices = c("I","II","III","IV","V","VI","VII","VIII","IX","X","XI","XII","XIII","XIV","XV","XVI", "Mito"))
# })
#
# group <- reactive({ paste( study, input$chrom, sep="/" ) })
#
# sampleData <- reactive({ sd = getSampleData( tallyFile, group() ); sd$Sample = c("YB210","s288c"); sd })
#
# variants <- reactive({
# subset( variantCalls, seqnames == input$chrom & AF >= input$af[1] & AF <= input$af[2] & Support >= input$minSupport & Coverage >= input$minCoverage )
# })
#
# output$variantSelect <- renderUI({
# tmp = seq(nrow(variants()))
# names(tmp) = paste( variants()$start, " - ", variants()$refAllele, "/", variants()$altAllele, sep="" )
# selectInput( "var", "Variant:", choices = tmp )
# })
#
# pos <- reactive({
# variants()$start[as.numeric(input$var)]
# })
#
# data <- reactive({
# if( nrow(variants()) > 0 ){
# h5dapply(
# filename = tallyFile,
# group = group(),
# blocksize = input$windowsize*3,
# names = c("Coverages","Counts","Deletions"),
# range = c( max( pos() - input$windowsize, 0 ), min( pos() + input$windowsize, chromlengths[input$chrom] ) )
# )[[1]]
# }else{
# NULL
# }
# })
#
# output$mismatchPlot <- renderPlot({
# if( nrow(variants()) > 0 ){
# p = mismatchPlot(
# data(),
# sampleData(),
# samples = c("s288c","YB210"),
# input$windowsize,
# pos()
# )
# print(p)
# }
# })
#
# output$variantTable <- renderTable({
# variants()[,c("seqnames", "start", "refAllele", "altAllele", "AF", "Support", "Coverage")]
# })
#
# output$afHist = renderPlot({
# hist( variants()$AF, breaks = seq(0,1,0.01) )
# })
#
# })
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