findAndTrimSeq: Find and trim a short pattern sequence from the subject.

In hiReadsProcessor: Functions to process LM-PCR reads from 454/Illumina data

Description

This function facilitates finding and trimming of a short pattern sequence from a collection of subject sequences. The trimming is dictated by side parameter. For more information on the trimming process see the 'side' parameter documentation in trimSeqs. For information regarding the pattern alignment see the documentation for pairwiseAlignSeqs. This function is meant for aligning a short pattern onto large collection of subjects. If you are looking to align a vector sequence to subjects, then please use BLAT.

Usage

findAndTrimSeq(patternSeq, subjectSeqs, side = "left", offBy = 0,
  alignWay = "slow", ...)

Arguments

patternSeq	DNAString object or a sequence containing the query sequence to search.
subjectSeqs	DNAStringSet object containing sequences to be searched for the pattern.
side	which side of the sequence to perform the search & trimming: left, right or middle. Default is 'left'.
offBy	integer value dictating if the trimming base should be offset by X number of bases. Default is 0.
alignWay	method to utilize for detecting the primers. One of following: "slow" (Default), "fast", or "blat". Fast, calls vpairwiseAlignSeqs and uses vmatchPattern at its core, which is less accurate with indels and mismatches but much faster. Slow, calls pairwiseAlignSeqs and uses pairwiseAlignment at its core, which is accurate with indels and mismatches but slower. Blat will use blatSeqs.
...	parameters to be passed to pairwiseAlignment, vpairwiseAlignSeqs or blatSeqs depending on which method is defined in 'alignWay' parameter.

Value

DNAStringSet object with pattern sequence removed from the subject sequences.

Note

If parallel=TRUE, then be sure to have a parallel backend registered before running the function. One can use any of the following MulticoreParam SnowParam

See Also

pairwiseAlignSeqs, vpairwiseAlignSeqs, extractFeature, extractSeqs, primerIDAlignSeqs, findPrimers, findLinkers

Examples

findAndTrimSeq(patternSeq="AGACCCTTTT",
subjectSeqs=DNAStringSet(c("AGACCCTTTTGAGCAGCAT","AGACCCTTGGTCGACTCA",
"AGACCCTTTTGACGAGCTAG")), qualityThreshold=.85, doRC=FALSE, side="left", 
offBy=1, alignWay = "slow")

hiReadsProcessor documentation built on Nov. 8, 2020, 5:43 p.m.