exp3_OHT_ESR1: exp3_OHT_ESR1 dataset
In qPLEXanalyzer: Tools for qPLEX-RIME data analysis
Description
Three ER qPLEX-RIME (10plex) experiments were performed to investigate the
dynamics of the ER complex assembly upon 4-hydrotamoxifen (OHT) treatment at
2h, 6h and 24h or at 24h post-treatment with the drug-vehicle alone
(ethanol). Two biological replicates of each condition were included in each
experiment to finally consider a total of six replicates per time point.
Additionally, MCF7 cells were treated with OHT or ethanol and cross-linked
at 24h post-treatment in each experiment to be used for mock IgG pull-downs
and to enable discrimination of non-specific binding in the same experiment.
This is a timecourse experiment to study the effect of tamoxifen in ER
interactome using qPLEX-RIME method.
Format
An object of class list related to peptides
quantification. It consists of qPLEX-RIME data from three experimental runs.
Each run contains 10 samples divided into five conditions (IgG, vehicle,
tam.2h, tam.6h and tam.24h).
Value
An object of class list related to peptides
quantification.
qPLEXanalyzer documentation built on Feb. 3, 2021, 2:01 a.m.
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Description
Three ER qPLEX-RIME (10plex) experiments were performed to investigate the dynamics of the ER complex assembly upon 4-hydrotamoxifen (OHT) treatment at 2h, 6h and 24h or at 24h post-treatment with the drug-vehicle alone (ethanol). Two biological replicates of each condition were included in each experiment to finally consider a total of six replicates per time point. Additionally, MCF7 cells were treated with OHT or ethanol and cross-linked at 24h post-treatment in each experiment to be used for mock IgG pull-downs and to enable discrimination of non-specific binding in the same experiment. This is a timecourse experiment to study the effect of tamoxifen in ER interactome using qPLEX-RIME method.
Format
An object of class list related to peptides
quantification. It consists of qPLEX-RIME data from three experimental runs.
Each run contains 10 samples divided into five conditions (IgG, vehicle,
tam.2h, tam.6h and tam.24h).
Value
An object of class list related to peptides
quantification.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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