getAllSub: Identify all substitutions observed across genomic positions...

In wavClusteR: Sensitive and highly resolved identification of RNA-protein interaction sites in PAR-CLIP data

Description

All substitutions observed across genomic positions exhibiting user-defined minimum coverage are extracted and a count table is returned. This function supports parallel computing.

Usage

getAllSub(sortedBam, minCov = 20, cores = 1)

Arguments

sortedBam	GRanges object containing aligned reads as returned by readSortedBam
minCov	An integer defining the minimum coverage required at a genomic position exhibiting a substitution. Genomic positions of coverage less than minCov are discarded. Default is 20 (see Details).
cores	An integer defining the number of cores to be used for parallel processing, if available. Default is 1.

Details

The choice of the minimum coverage influences the variance of the relative substitution frequency estimates, which in turn affect the mixture model fit. A conservative value depending on the library size is recommended for a first analysis. Values smaller than 10 have not been tested and are therefore not recommended.

Value

A GRanges object containing a count table, where each range correspond to a substitution. The metadata correspond to the following information:

substitutions	observed substitution, e.g. AT, i.e. A in the reference sequence and T in the mapped read.
coverage	strand-specific coverage.
count	number of strand-specific substitutions.

Author(s)

Federico Comoglio and Cem Sievers, with contributions from Martin Morgan

See Also

readSortedBam

Examples

filename <- system.file( "extdata", "example.bam", package = "wavClusteR" )
example <- readSortedBam(filename = filename)
countTable <- getAllSub( example, minCov = 10, cores = 1 )
countTable

wavClusteR documentation built on Nov. 8, 2020, 6:54 p.m.