TwoPrimerRemove: Clean biological secuences
In CleanBSequences: Curing of Biological Sequences

TwoPrimerRemove

R Documentation

Clean biological secuences

Description

Curates biological sequences of two restriction enzyme primers or cloning vectors.This cleaning is required for techniques such as cDNA-AFLP.

Usage

TwoPrimerRemove(SEQs, PrimerF, PrimerR)

Arguments

`SEQs`	DNAString containing biological sequences that are to be cleaned.
`PrimerF`	dnastring containing the foward primer/vector sequences to be removed.
`PrimerR`	dnastring containing the reverse primer/vector sequences to be removed.

Value

clean biological sequences and visualization of the alignments

Author(s)

Florencia I Pozzi, Silvina A. Felitti

Examples

SEQs = DNAString(paste("ACTTTCTGCTGCTTGTGGTCGCAATCAGAGTCCTGATGATGAGTCCTGA",
"CCGAACCCTTTTTCTCCGTCATCCGTTGGTCCATGGTACGCAATCAGAG", sep = ""))
PrimerF = DNAString("GATGAGTCCTGACCGAA")
PrimerR = DNAString("GACTGCGTACCATGC")
TwoPrimerRemove (SEQs,PrimerF,PrimerR)

CleanBSequences documentation built on April 28, 2022, 1:07 a.m.