Description Usage Arguments Details Value Author(s) References
Does similar procedures as cq2log, but in the end converts the data into relative expression values, leaves it unstacked, and removes factor columns.
1 |
data |
raw qRT-PCR data; see help for cq2counts for details on formatting |
genes |
Vector of names of the potential control genes. Make sure you select only the potential control genes for this analysis, otherwise geNorm might prefer actual responsive genes if they co-vary and have very low variance. |
effic |
PCR efficiency data for each of the analyzed genes; see help for cq2counts for details on formatting. |
noamp |
what to do about Ct values '-1', denoting no amplification. By default, these will be assigned an arbitrarily low expression value corresponding to slightly less than one molecule. Specify 'noamp=NA' if you want to skip samples containing any of these. |
See cq2counts help page for details.
A dataset to be fed into the function selectHKgenes (package SLqPCR).
Mikhail V. Matz, UT Austin
Matz MV, Wright RM, Scott JG (2013) No Control Genes Required: Bayesian Analysis of qRT-PCR Data. PLoS ONE 8(8): e71448. doi:10.1371/journal.pone.0071448
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