Description Usage Arguments Value Author(s) References
View source: R/normalize.qpcr.R
Performs multigene normalization as per Vandesompele et al 2002, centers the resulting gene expression values around the gene's means
1 | normalize.qpcr(data, controls, center = T)
|
data |
The output of cq2log() |
controls |
Vector of control genes, such as 'controls=c("eif3h","nd5","rpl11")' |
center |
Whether to perform centering around mean or not. |
normalized dataset
Mikhail Matz, UT Austin
Matz MV, Wright RM, Scott JG (2013) No Control Genes Required: Bayesian Analysis of qRT-PCR Data. PLoS ONE 8(8): e71448. doi:10.1371/journal.pone.0071448
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