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inst/app/www/about-fov.md
In NACHO: NanoString Quality Control Dashboard
Each individual lane scanned on an nCounter system is divided into a few hundred imaging sections, called Fields of View (FOV), the exact number of which will depend on the system being used (i.e., MAX/FLEX or SPRINT), and the scanner settings selected by the user.
The system images these FOVs separately, and sums the barcode counts of all FOVs from a single lane to form the final raw data count for each unique barcode target.
Finally, the system reports the number of FOVs successfully imaged as FOV Counted.
Significant discrepancy between the number of FOV for which imaging was attempted (FOV Count) and for which imaging was successful (FOV Counted) may indicate an issue with imaging performance.
Recommended percentage of registered FOVs (i.e., FOV Counted over FOV Count) is 75 %.
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NACHO documentation built on Aug. 8, 2023, 1:08 a.m.
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Each individual lane scanned on an nCounter system is divided into a few hundred imaging sections, called Fields of View (FOV), the exact number of which will depend on the system being used (i.e., MAX/FLEX or SPRINT), and the scanner settings selected by the user. The system images these FOVs separately, and sums the barcode counts of all FOVs from a single lane to form the final raw data count for each unique barcode target. Finally, the system reports the number of FOVs successfully imaged as FOV Counted.
Significant discrepancy between the number of FOV for which imaging was attempted (FOV Count) and for which imaging was successful (FOV Counted) may indicate an issue with imaging performance.
Recommended percentage of registered FOVs (i.e., FOV Counted over FOV Count) is 75 %.
Try the NACHO package in your browser
Any scripts or data that you put into this service are public.
R Package Documentation
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We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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