Description Usage Arguments Details
This function takes in a flowframe with debris removed and identifies ynechococcus cyanobacteria cell population in the provided frame.
1 | bs5_nc(bs4bs5, p1, p2, others, to_retain = "potential")
|
bs4bs5 |
flowframe with debris (left) removed. |
p1 |
first flowcytometer channel that can be used to separate cells of interest from the rest, e.g. "RED.B.HLin". |
p2 |
second flowcytometer channel that can be used to separate cells of interest from the rest, e.g. "YEL.B.HLin" |
others |
row numbers for non-debris events. This is provided by the debris_nc or debris_inc function. |
to_retain |
should potential candidates be retained or further gating be applied to filter out only certain Synechococcus cyanobacteria cells. @return list containing;
|
The function uses the getPeaks
and
deGate
functions in the flowDensity package to
identify peaks and identify cut-off points between these peaks.
This function is not designed to be called in isolation, if called
in isolation an error will be returned. It is preferably called on the results
from debris_nc
or debris_inc
function.
A graph with horizontal and vertical lines used in separating the populations
is returned and if to_retain="refined", a circle made of dashed lines is drawn around
Synechococcus cyanobacteria cell population points.
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