Description Usage Arguments Details Value See Also
The function takes in a flowframe and identifies debris contained in the provided flowframe.
1 | debris_nc(flowframe, p1, p2)
|
flowframe |
flowframe with debris and other cells. |
p1 |
first flowcytometer channel that can be used to separate debris from the rest, e.g. "RED.B.HLin". |
p2 |
second flowcytometer channel that can be used to separate debris from the rest, e.g. "YEL.B.HLin" |
The function uses the getPeaks
and
deGate
functions in the flowDensity package to
identify peaks between peaks and identify cut-off points between these peaks.
A plot of both channels supplied with horizontal line separating
debris from other cell populations is also returned.
list containing;
syn - flowframe containing non-debris particles
deb_pos - position of particles that are debris
syn_pos - position of particles that are not debris
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