Nothing
## ----include = FALSE----------------------------------------------------------
knitr::opts_chunk$set(
tidy = FALSE,
collapse = TRUE,
comment = "#>",
fig.width = 5,
fig.height = 4
)
## ----setup, message = FALSE---------------------------------------------------
##library
library(magrittr)
library(grid)
library(Seurat)
library(ggplot2)
library(ggsector)
## ----fig.width=3, fig.height=3------------------------------------------------
tmp_df <- sector_df(x = 0.5, y = 0.5, theta = 25, r = 0.4, start = 0, r_start = 0)
head(tmp_df)
grid.newpage()
grid.polygon(
tmp_df$x, tmp_df$y,
vp = viewport(height = unit(1, "snpc"), width = unit(1, "snpc"))
)
## ----fig.width=3, fig.height=3------------------------------------------------
tmp_df <- sector_df(x = 0.5, y = 0.5, theta = 25, r = 0.4, start = 50, r_start = 0.2)
head(tmp_df)
grid.newpage()
grid.polygon(
tmp_df$x, tmp_df$y,
vp = viewport(height = unit(1, "snpc"), width = unit(1, "snpc"))
)
## ----fig.width=3, fig.height=3------------------------------------------------
tmp_df <- sector_df(
x = 0.5, y = 0.5, theta = 180, r = 0.4,
start = 90, r_start = 0, type = "degree"
)
head(tmp_df)
grid.newpage()
grid.polygon(
tmp_df$x, tmp_df$y,
vp = viewport(height = unit(1, "snpc"), width = unit(1, "snpc"))
)
## ----fig.width=3, fig.height=3------------------------------------------------
tmp_df <- sector_df(
x = 0.5, y = 0.5, theta = 180, r = 0.4,
start = 270, r_start = 0.2, type = "degree"
)
head(tmp_df)
grid.newpage()
grid.polygon(
tmp_df$x, tmp_df$y,
vp = viewport(height = unit(1, "snpc"), width = unit(1, "snpc"))
)
## ----fig.width=3, fig.height=3------------------------------------------------
tmp_df <- sector_df_multiple(
x = c(0.2, 0.5, 0.8),
theta = c(25, 50, 75),
r = 0.15,
start = c(75, 50, 100),
r_start = c(0, 0.05, 0.1),
type = "percent"
)
head(tmp_df)
grid.newpage()
grid.polygon(
tmp_df$x,
tmp_df$y,
id = tmp_df$group,
vp = viewport(height = unit(1, "snpc"), width = unit(1, "snpc")),
gp = gpar(
fill = 3:1, col = 1:3
)
)
## -----------------------------------------------------------------------------
grid.newpage()
gp <- sectorGrob(
x = unit(c(3, 5, 7), "cm"),
y = unit(c(3, 5, 7), "cm"),
theta = c(90, 180, 270),
r = 1,
start = c(180, 180, 270),
r_start = c(0.6, 0.3, 0),
type = "degree",
group = factor(1:3, levels = c(2, 3, 1)),
gp = gpar(fill = c("green", "red", "blue"))
)
grid.draw(gp)
## -----------------------------------------------------------------------------
grid.newpage()
grid.sector(
x = c(0.1, 0.5, 0.9),
y = c(0.9, 0.6, 0.1),
theta = c(25, 50, 90),
r = .1,
start = c(25, 50, 100),
r_start = c(0.06, 0.03, 0),
type = "percent",
group = factor(1:3, levels = c(2, 3, 1)),
gp = gpar(col = c("green", "red", "blue"), fill = 2:4),
default.units = "npc"
)
## ----eval=rlang::is_installed("ComplexHeatmap")-------------------------------
## install ComplexHeatmap
# if (!require("ComplexHeatmap", quietly = TRUE)) {
# if (!require("BiocManager", quietly = TRUE)) {
# install.packages("BiocManager")
# }
# BiocManager::install("ComplexHeatmap")
# }
## run
library(magrittr)
library(ComplexHeatmap)
t0 <- cor(mtcars) %>%
set_colnames(paste("y_", colnames(.))) %>%
set_rownames(paste("x_", rownames(.)))
mat <- abs(t0)
mat[1:5, 1:5]
## ----fig.width=8, fig.height=8, eval=rlang::is_installed("ComplexHeatmap")----
set.seed(1)
Heatmap(
mat,
name = "vp",
rect_gp = gpar(type = "none"),
cell_fun = function(j, i, x, y, width, height, fill) {
grid.rect(
x = x, y = y, width = width, height = height,
gp = gpar(col = "grey", fill = NA)
)
grid.sector(
theta = mat[i, j] * 100,
r = 0.5,
start = mat[i, j] * 100 * runif(1),
r_start = mat[i, j] * 0.49 * runif(1),
vp = viewport(x, y, width, height),
gp = gpar(fill = fill, col = "transparent")
)
},
width = unit(.7, "snpc"),
height = unit(.7, "snpc")
)
## ----fig.width=8, fig.height=8, eval=rlang::is_installed("ComplexHeatmap")----
# The default viewport locks the horizontal and vertical axes
# so that the sector does not deform, which needs to be removed here.
# The radius 'r' is half the min(length, width).
set.seed(2)
Heatmap(
mat,
name = "xy + r",
rect_gp = gpar(type = "none"),
cell_fun = function(j, i, x, y, width, height, fill) {
grid.rect(
x = x, y = y, width = width, height = height,
gp = gpar(col = "grey", fill = NA)
)
r <- as.numeric(min(width, height)) / 2
grid.sector(
x,
y,
theta = mat[i, j] * 100,
r = r,
start = mat[i, j] * 100 * runif(1),
r_start = mat[i, j] * r * 0.9 * runif(1),
vp = NULL,
gp = gpar(fill = fill, col = "transparent")
)
},
width = unit(.7, "snpc"),
height = unit(.7, "snpc")
)
## ----fig.width=8, fig.height=8, eval=rlang::is_installed("ComplexHeatmap")----
# The input matrix needs to be extracted with pindex(mat, i, j)
set.seed(3)
Heatmap(
mat,
name = "layer",
rect_gp = gpar(type = "none"),
layer_fun = function(j, i, x, y, width, height, fill) {
grid.rect(
x = x, y = y, width = width, height = height,
gp = gpar(col = "grey", fill = NA)
)
r <- as.numeric(min(width, height)) / 2
grid.sector(
x,
y,
theta = pindex(mat, i, j) * 100,
r = r,
start = pindex(mat, i, j) * 100 * runif(nrow(mat) * ncol(mat)),
r_start = pindex(mat, i, j) * r * 0.9 * runif(nrow(mat) * ncol(mat)),
vp = NULL,
gp = gpar(fill = fill, col = "transparent")
)
},
width = unit(.7, "snpc"),
height = unit(.7, "snpc")
)
## -----------------------------------------------------------------------------
library(ggsector)
library(reshape2)
df <- cor(mtcars)[1:3, 1:5] %>%
abs() %>%
melt(varnames = c("x", "y"))
## Note, for better display effect, please always add coord_fixed()
## Note, for better display effect, please always add coord_fixed()
## Note, for better display effect, please always add coord_fixed()
## ----fig.width=5, fig.height=4------------------------------------------------
ggplot(df) +
## type = "percent", theta = 0-100
geom_sector(
aes(y, x, theta = value * 100),
type = "percent",
color = "blue",
individual = TRUE
) +
## type = "degree", theta = 0-360
geom_sector(
aes(y, x, theta = value * 360),
type = "degree",
color = "red",
alpha = 0.5,
individual = TRUE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## -----------------------------------------------------------------------------
ggplot(df) +
geom_sector(
aes(y, x, theta = value * 100),
r = rep(c(0.15, 0.3, 0.45), 5),
fill = 2,
individual = TRUE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## -----------------------------------------------------------------------------
ggplot(df) +
geom_sector(
aes(y, x, theta = value * 100),
start = rep(c(60, 40, 20), 5),
fill = 2,
individual = TRUE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## -----------------------------------------------------------------------------
ggplot(df) +
geom_sector(
aes(y, x, theta = value * 100),
r_start = rep(c(0.15, 0.25, 0.35), 5),
fill = 2,
individual = TRUE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=6, fig.height=6------------------------------------------------
# x = x, y = y
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(x, y),
theta = 75,
fill = 2,
r = 0.5,
individual = TRUE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=8, fig.height=3------------------------------------------------
# x = y, y =x
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(y, x),
theta = 75,
fill = 2,
r = 0.5,
individual = TRUE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=6, fig.height=6------------------------------------------------
# x = x, y = y
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(x, y),
theta = 75,
fill = 2,
r = 0.5,
individual = FALSE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=8, fig.height=3------------------------------------------------
# x = y, y =x
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(y, x),
theta = 75,
fill = 2,
r = 0.5,
individual = TRUE
) +
coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=6, fig.height=4------------------------------------------------
# x = x, y = y
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(x, y),
theta = 75,
fill = 2,
r = 0.35, ## To reduce the radius, you need to try it manually
individual = TRUE
) +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=6, fig.height=4------------------------------------------------
# x = y, y =x
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(y, x),
theta = 75,
fill = 2,
r = 0.25, ## To reduce the radius, you need to try it manually
individual = TRUE
) +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=6, fig.height=4------------------------------------------------
# x = x, y = y
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(x, y),
theta = 75,
fill = 2,
r = 0.5,
## You need to manually adjust the `ratio` value
## to prevent sector deformation.
ratio = 1.6,
individual = FALSE
) +
theme_bw() +
theme(axis.title = element_blank())
## ----fig.width=6, fig.height=4------------------------------------------------
# x = y, y =x
ggplot(rbind(
cbind(df, t1 = 1),
cbind(df[1:9, ], t1 = 2)
)) +
facet_wrap(~t1, ncol = 2) +
geom_sector(
aes(y, x),
theta = 75,
fill = 2,
r = 0.5,
## You need to manually adjust the `ratio` value
## to prevent sector deformation.
ratio = 1.6,
individual = FALSE
) +
# coord_fixed() +
theme_bw() +
theme(axis.title = element_blank())
## ----eval = FALSE-------------------------------------------------------------
# ## Download pbmc data from
# # https://cf.10xgenomics.com/samples/cell/pbmc3k/pbmc3k_filtered_gene_bc_matrices.tar.gz
# library(Seurat)
# path <- paste0(tempdir(), "/pbmc3k.tar.gz")
# file <- paste0(tempdir(), "/filtered_gene_bc_matrices/hg19")
# download.file(
# "https://cf.10xgenomics.com/samples/cell/pbmc3k/pbmc3k_filtered_gene_bc_matrices.tar.gz",
# path
# )
# untar(path, exdir = tempdir())
# pbmc.data <- Read10X(data.dir = file)
# pbmc <- CreateSeuratObject(
# counts = pbmc.data, project = "pbmc3k",
# min.cells = 3, min.features = 200
# )
# pbmc <- NormalizeData(pbmc)
# pbmc <- FindVariableFeatures(pbmc, selection.method = "vst", nfeatures = 2000)
# pbmc <- ScaleData(pbmc, features = rownames(pbmc))
# pbmc <- RunPCA(pbmc)
# pbmc <- RunUMAP(pbmc, dim = 1:10)
# pbmc <- FindNeighbors(pbmc, dims = 1:10)
# pbmc <- FindClusters(pbmc, resolution = 1)
# pbmc <- FindClusters(pbmc, resolution = 0.5)
# markers <- tibble::tribble(
# ~type, ~marker,
# "Naive CD4+ T", "IL7R,CCR7",
# "CD14+ Mono", "CD14,LYZ",
# "Memory CD4+", "IL7R,S100A4",
# "B", "MS4A1",
# "CD8+ T", "CD8A",
# "FCGR3A+ Mono", "FCGR3A,MS4A7",
# "NK", "GNLY,NKG7",
# "DC", "FCER1A,CST3",
# "Platelet", "PPBP",
# ) %>%
# tidyr::separate_rows(marker, sep = ", *") %>%
# dplyr::distinct()
#
# # Dotplot
# DotPlot(pbmc, features = unique(markers$marker)) + coord_flip()
#
# # contrast with DotPlot
# SectorPlot(pbmc, markers$marker, features.level = unique(rev(markers$marker)))
#
# SectorPlot(pbmc, markers$marker, group.by = "RNA_snn_res.1")
# SectorPlot(pbmc, markers$marker, group.by = "RNA_snn_res.1", slot = "scale.data")
#
# # split plot
# # Assume a variable 'day', expressed as the number of days of cell development.
# set.seed(1)
# pbmc[["day"]] <- sample(1:3, ncol(pbmc), TRUE)
# SectorPlot(pbmc, markers$marker, group.by = "RNA_snn_res.0.5", split.by = "day")
# SectorPlot(
# pbmc, markers$marker,
# group.by = "day", split.by = "RNA_snn_res.0.5", nrow = 1
# )
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